摘要
基于色氨酸和半胱氨酸对Ru（pheo）3^2＋-Ce（Ⅳ）体系化学发光的增强作用及其发光动力学性质的显著差别，提出了一种化学发光法同时测定这两种氨基酸的新方法。在优化的实验条件下，该方法测定色氨酸和半胱氨酸的线性范围分别为0．05～2．0μg／mL和0．05～3．0μg／mL，检出限分别为0．03μg／mL和0．02μg/mL。将其应用于合成样品中两种氨基酸的同时测定。结合文献提出了可能的化学发光反应机理。
It was observed that both tryptophan and cysteine could enhance the chemiluminescence （CL） intensity of tris-（1,10-phenanthroline） ruthenium （ Ⅱ ） （Ru（phen）3^2＋ ） - Ce（Ⅳ） system and their CL kinetics curves were remarkably different. Based on above phenomena, a novel CL method was proposed for the simultaneous determination of tryptophan and cysteine. Under the optimum experimental conditions, the linear range and detection limit for tryptophan were 0.05 - 2.0μg/mL and 0.03μg/mL, respectively; and 0.05 - 3.0μg/mL and 0.02μg/mL for cysteine, respectively. The proposed method has been used to analyze the synthetic samples. The possible mechanism of CL reaction was studied.
出处
《分析试验室》
CAS
CSCD
北大核心
2007年第7期5-8,共4页
Chinese Journal of Analysis Laboratory
关键词
色氨酸
半胱氨酸
化学发光
同时测定
Tryptophan
Cysteine
Chemiluminescence
Simultaneous determination
