摘要
目的建立稳定的模拟人类颈动脉粥样硬化性狭窄的动物模型。方法普通猪10头,采用血管内膜热空气损伤法在猪的颈总动脉上造成特定条件的损伤,然后以特定高脂饲料喂养2个月,评价猪血管狭窄程度和病理改变特点。结果实验动物无死亡,共20根血管有19根血管狭窄率≥70%,病理检查证实高脂喂养2个月后颈动脉的粥样硬化病理改变已属于较成熟的纤维斑块期。结论猪的颈动脉与人类相似程度高,按本实验方法,损伤后喂养2个月的猪颈动脉粥样硬化性狭窄程度和病理改变均较好符合颈动脉狭窄研究之实验需要。
Objective To discuss the methods and factors influencing the development of a stable and rehable animal model suitable for experimental studies of surgical treatments of carotid atherosclerotic stenosis （CASS）. Methods In 10 normal pigs ,lesions were created by hot air-drying isolated segments of carotid arteries and feeding the pigs a diet of 6% cholesterol, 12% peanut oil for 2 months. Digital subtraction angiography was used to evaluate the stenosis of carotid arteries and observe the characters of pathological changes. Results No animal was dead. In 20 blood vessels, 19 had stenosis rate of more than 70%. The pathological studies suggested that the atherosclerotic lesions were at the mature fibrous plaque period after feeding the pigs with the atherogenic diet for 2 months. Conclusion The carotid arteries of pigs have a well same as mankind in antatimical and physiology. Injured and fed as described by the authors, the percentage of stenosis and pathological changes of the animal model is stable and reliable, and it meets the demand of experimental studies of surgical treatment of CASS.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2007年第7期881-882,共2页
Chinese Journal of Experimental Surgery
关键词
颈动脉狭窄
粥样硬化
动物模型
猪
Carotid stenosis
Atherosclerosis
Model, animal
Pig
