摘要
目的:研究人工合成mutacinⅠ和Ⅲ对牙菌斑主要致龋菌的抑制作用,寻找其活性基团。方法:通过固相合成法合成mutacinⅠ和Ⅲ及不同链长的系列短肽。采用二倍稀释法检测系列短肽对变形链球菌和血链球菌的最小抑菌浓度,并检测其对变形链球菌、血链球菌、韦荣氏菌、黏性放线菌、内氏放线菌、远缘链球菌的抑菌圈直径大小。结果:人工合成mutacinⅠ和Ⅲ及部分短肽类似物对变形链球菌、远缘链球菌、血链球菌有抑菌活性。结论:人工合成的mutacinⅠ和Ⅲ及部分短肽类似物有一定的抑菌活性,可初步确定其活性基团位置。
AIM： To study the antimicrobial activity of peptide mutacin Ⅰ and Ⅲ and short peptide and detect the active group in vitro. METHODS： The peptide mutacin from S. mutans was synthesized by Merrifold peptide synthesor. Its amino acid sequence was detected by Fast technique. The antibiotic activities of peptide mutacin Ⅰ and Ⅲ and short peptide against Streptococcus mutaus, Streptococcus sobrinus, Streptococcus sanguis, Actinomyces naeslundi, Actinomyces viscosus were tested by drug sensitive slips method and double dilution method. RESULTS： The peptide mutacin Ⅰ and Ⅲ and short peptides only have bactericidal activity against Streptococcus mutaus, Streptococcus sobrinus and Streptococcus sanguis. CONCLUSIONS： The peptide mutacin Ⅰ and Ⅲ and some of short peptides have bactericidal activity. Some short peptides may be the active group.
出处
《牙体牙髓牙周病学杂志》
CAS
2007年第6期333-336,共4页
Chinese Journal of Conservative Dentistry
基金
陕西省科学技术研究发展计划项目资助[2004K11-G3(3)]
