摘要
目的探讨佛手茎尖微嫁接技术,为佛手无病苗木的培育奠定基础。方法黑暗条件下培养柠檬实生苗用作砧木,以佛手茎尖为接穗,采用倒"T"字形法进行茎尖微嫁接试验,并对影响嫁接成活率的因素进行研究。结果成功获得了佛手茎尖微嫁接苗,并优化了微嫁接的条件:选取苗龄为14d的柠檬实生苗为砧木,嫁接成活率较高;以带4个叶原基的佛手茎尖为接穗,效果较好,嫁接成活率可达65.2%;茎尖微嫁接后,切口外缠parafilm膜,能明显提高成活率。结论建立了佛手茎尖微嫁接的方法,获得了嫁接成活苗。
Objective To study the technique for shoot-tip grafting of Citrus medica var. sarcodactylis in order to lay a foundation for culturing disease-free plantlets. Methods Using dark grown lemon seedling as rootstock and shoot-tip of C. medica var. sarcodactylis as scion, the shoot-tip grafting was executed by grafting a scion in an inverted-T incision of lemon seedling. And the factors affecting the survival rate of shoot-tip grafting were studied. Results The micro-grafted plantlets of C. medica var. sarcodactylis were achieved. It was suggested that the more survival rate of grafting was obtained by using 14- day-old dark grown seedlings as rootstocks. The scions consisted of the apical meristem plus four leaf primordia were optimal for grafting, with survival rate of 65.2%. Moreover, the survival rate of grafting could be increased obviously if the grafting-incisions were twisted with parafilm. Conclusion A procedure of shoot-tip grafting in vitro has been established in C. medica var. sarcodactylis and micro-grafted survival plantlets have been obtained.
出处
《中草药》
CAS
CSCD
北大核心
2007年第7期1081-1084,共4页
Chinese Traditional and Herbal Drugs
基金
广东省自然科学基金项目(020792)
国家中医药管理局研究课题(02-03ZP48)
关键词
佛手
茎尖微嫁接
成活率
Citrus medica L. var. sarcodactylis （Noot.） Swingle
shoot-tip grafting
survival rate
