摘要
目的提高超临界CO2萃取人参中人参皂苷的萃取率。方法采用二-(2-乙基己基)磺基琥珀酸钠(AOT)/乙醇/水/超临界CO2反相微乳对人参皂苷的萃取进行研究。结果在萃取压力25MPa、温度45℃、时间4h、CO2流量为2L/h条件下,超临界CO2反相微乳萃取的人参皂苷萃取率是乙醇/水/超临界CO2萃取的3.2倍。人参皂苷的萃取率随加入的水量、萃取压力的增大而增大,随AOT浓度、萃取温度的升高先增大后减小。萃取人参皂苷时,采用适量水先浸泡物料与萃取过程中加入水相比,人参皂苷的萃取率要提高30%。结论结合实验结果与理论探讨,超临界CO2反相微乳萃取人参皂苷的机制主要是其形成的极性水池增大了对人参皂苷的溶解度。
Objective To raise ginsenosides yield from Panax ginseng by supercritical CO2 reverse microemulsion extraction. Methods Bis-（2-ethylhexyl） sodium sulphosuccinate （AOT）/ethanol/water/supercritical CO2 reverse microemulsion extraction was carried out to extract gingsenosides. Results The ginsenosides extracting rate by supercritical CO2 reverse microemulsion extraction was 3.2 times that of by ethanol/water/supercritical CO2 extraction in extracting pressure 25 MPa, extracting temperature 45℃,extracting time 4 h, and COz flow rate 2.0 L/h, respectively. The ginsenosides extracting yield increased with the increasing of the water amount and the extracting pressure, increased first and then decreased with the increasing of AOT concentration and the extracting temperature. The ginsenosides extracting yield with P. ginseng soaked with water before extraction was 1.3 times that with P. ginseng soaked with water during the extraction. Conclusion Combined the experimental results with the theoretic deduction, the mechanism of supercritical CO2 reverse microemulsion extraction is that the polar water pools of reverse microemulsion can make more ginsenosides dissolved.
出处
《中草药》
CAS
CSCD
北大核心
2007年第7期1003-1006,共4页
Chinese Traditional and Herbal Drugs
关键词
人参
人参皂苷
超临界CO2
反相微乳萃取
超临界CO2萃取
Panax ginseng C. A. Meyer
ginsenosides
supercritical CO2 reverse microemulsion extraction
supercritical CO2 extraction
