摘要
目的建立化学发光酶联免疫分析法（CLEIA）检测肾综合征出血热（HFRS）患者血清中IgM抗体。方法以抗人IgM-μ链抗体包被黑色不透明聚乙烯板，辣根过氧化物酶标记汉坦病毒核蛋白作为检测抗原以及luminol-H2O2作为发光底物，建立CLEIA法并对CLEIA与IgM抗体捕获酶联免疫吸附法（MacELISA）进行比较。结果CLEIA与MacELISA相关系数0．97；对于51份确诊的HFRS患者急性期血清CLEIA检测敏感度100％，MacELISA为90．2％；对48份正常人血清两种方法检测特异度均为100％；CLEIA次内变异系数范围5．02％-12．7％，次间变异系数范围0．4％～7．0％，与MacELISA相当。结论化学发光酶联免疫分析法是一种更为灵敏，准确和稳定的方法，适用于检测HFRS早期患者血清中IgM抗体。
Objective To development a chemiluminescent enzyme-linked immunoassay（CLEIA） for the detection of HTNV IgM antibody. Methods Black solid 96 well microplate was coated with anti-human IgM-μ antibody, HRP labeled HTNV recombinant nucleotide antigen was used as detection antigen, luminol-H2O2 was used as substrate, a CLEIA was established for the detection of HFRS patient serum IgM antibody and comparison of detection sensitivity, specificity, and stability were made between CLEIA and MacELISA. Results Correlate coefficient of CLEIA with MacELISA is 0.97; detection sensitivity of CLEIA is 100% while that of MacELISA is 90.2% ; detection specificity of CLEIA and MacELISA are both 100% ; coefficient of variance for intra-assay and interassay of CLEIA are both less than 15%, which are comparative with MacELISA. Conclusion The established method of CLEIA is a sensitive, selective, and stable method, it is suitable for the early detection of HFRS patient serum IgM antibody.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2007年第2期171-173,共3页
Chinese Journal of Experimental and Clinical Virology
关键词
肾综合征出血热
辣根过氧化物酶
化学发光
酶联免疫吸附测定
免疫球蛋白M
Hemorrhagic fever with renal Syndome
Horseradish peroxidase
Chemiluminescencet
Enzyme-linked immunosorbent assay
Immunoglobulin M
