摘要
目的特发性无精症及严重少精子症患者Y染色体上DAZ基因缺失发生情况。方法采用聚合酶链方法(STS-PCR)对38例无精子症及严重少精子症患者Y染色体上DAZ基因上的4个位点SY154?SY155?SY254?SY255及SRY基因进行检测。结果38例患者中共检出6例DAZ基因缺失,缺失率15.8%,其中30例特发性无精子症患者中有5例缺失,缺失率16.7%,8例严重少精子症患者中有1例缺失,缺失率12.5%。结论DAZ基因的缺失是引起无精子症和严重少精子造成男性不育的重要原因之一,对特发性无精子和严重少精子的不育男性,在进行卵细胞质内精子注射技术(ICSI)前应进行DAZ的检测并行移植前诊断(PGD)。
Objective To evaluate microdeletion of DAZ gene on the Y chromosome in patients suffering from idiopathic azoospermia or severe oligozoospermia. Methods The four sites including SY154,SY155,SY254,SY255 and SRY regions of Y chromosome in 38 cases of idiopathic azoospermia and severe oligozoospermia were detected by STS - PCR. Results Six cases of microdeletion of DAZ genes were found in 38 patiens,microdeletion rate was 15.8%. Five cases from 30 patiens suffering from idiopathic azoospermia,microdeletion rate was 16.7%. One case from 8 patients suffering from severe oligozoospermia had microdeletion. The microdeletion rate was 12.5%. Conclusions The microdeletion of DAZ gene is a major cause of idiopathic azoospermia and severe oligozoospermia leading to male infertility. Therefore, DAZ gene should be detected and PGD should be done prior to ICSI for the patients suffering from idiopathic azoospermia and severe oligozoospermia.
出处
《医学研究杂志》
2007年第7期56-58,共3页
Journal of Medical Research
