摘要
目的研究脐血干细胞向巨核细胞分化后信号转导基因表达的改变。方法分别收集同一份脐血分化培养前CD34+细胞和培养后CD41+细胞,提取总RNA。用基因芯片技术比较两组之间的基因表达差异,并用RT-PCR技术验证芯片结果。结果芯片分析结果显示,共筛选出3522个差异基因,其中上调1705个,下调1817个。3522个差异基因中,与细胞信号相关的基因有343个,与转录调节相关的有150个,与分化相关的有21个。其中,CD61基因的表达增加了369.83倍,CD41基因的表达增加27.38倍,PF4基因的表达增加24.06倍;MAPKs、GPCRs(G蛋白偶联受体)、RAS家族相关的基因多数表达上调;与STAT通路相关的基因中,SOCS1、JAK2表达上调,STAT5A表达下调。结论TPO等造血生长因子可能主要通过GPCRs-Ras-MAPK途径,促进脐血干细胞向巨核细胞系增殖、分化。
Objective To study the alterations of gene expression after stem cells in cord blood differentiate into megakaryocyte. Methods RNA was extracted from CD34＋ cells before culture and CD41＋ cells after culture of the same cord blood, and analyzed the differential expression genes by gene chip. RT-PCR was employed to validate the results of gene chip. Results 3 522 differentially expressed genes were screened by gene chip, including 1 705 up-regulated genes and 1 817 down-regulated genes. There were 343 genes involved in cell signals, 150 genes involved in transcription regulator, 21 genes involved in differenciation. The expression of CD61 gene increased 369.83 folds, the expression of CD41 gene increased 27.38 folds, and the expression of PF4 gene increased 24. 06 folds. The expression of MAPKs, GPCRs （G protein-coupled receptors） and members of RAS oncogene family increased mostly; the expression of the genes involved in STAT pathway, both SOCS1 and JAK2 up-regulated, but STAT5A down-regulated. Conclusion TPO and some other hemal;opoietic growth factors may enhance the proliferation and differentiation of megakaryocytes derived from stem cells in cord blood by GPCRs-Ras-MAPK pathway.
出处
《福建医药杂志》
CAS
2007年第1期1-4,共4页
Fujian Medical Journal
基金
福建省自然科学基金资助项目(c0210026)
关键词
脐血
干细胞
巨核细胞
基因芯片
基因表达
Cord bloods Stem cell
Megakaryocyte
Gene chip
Gene expression
