摘要
分支杆菌、尤其是副结核杆菌长期被疑为克隆病的致病菌。采用聚合酶链反应技术对手术及内镜活检的７４例石蜡包埋组织中的副结核杆菌ＤＮＡ进行检测。扩增的靶ＤＮＡ为副结核杆菌染色体特异重复插入序列ＩＳ９００１上４００ｂｐ的片段。其产笺特异性通过生物素杆主民的副结核杆菌全染色体探针Ｓｏｕｔｈｅｒｎ杂交证实。
Crohn′s disease has long been suspected of having a mycobacteria, specifically M. paratuberculosis cause. In this study polymerase chain reaction (PCR) assay was used to detect M. paratuberculosis DNA in 74 paraffin wax embedded gut tissues from endoscopic biopsy or surgical resection specimens, including 36 Crohn′s disease tissues, 18 ulcerative colitis tissues and 20 non inflammatory bowel disease tissues. Oligonucleotides derived from IS900 sequeuce, which is a repeated in M. paratuberculosis chromosome and highly specific for the M. paratuberculosis, were used as primer. The specificity of products was comfirmed by southern blot hybridization by using a biotin labeled probe. The results show that M. paratuberculosis DNA was detected in 17 Crohn′s disease (47.2%), 2 ulcerative colitis (11.1%) and 3 non inflammatory bowel disease control subjects (15.0%). PCR positive or negative results in 36 Crohn′s disease show that there is no relation with small or large gut involvement, and also the presence or absence of granulomata. The results suggest that M. paratuberculosis is surely present in tissues from some patients with Crohn′s disease, which may have a specific association with Crohn′s disease.
出处
《中华内科杂志》
CAS
CSCD
北大核心
1997年第4期228-230,共3页
Chinese Journal of Internal Medicine
基金
四川省卫生厅科学研究基金
关键词
克隆病
副结核杆菌
聚合酶链反应
Mycobacterium paratuberculosis Crohn disease Polymerase chain reaction
