摘要
背景与目的:TNF、某些化疗药物以及电离辐射可以活化NF-κB,从而抑制细胞凋亡。NF-κB的活化可以通过特异性抑制剂吡咯二硫氨基甲酸脂(pyrrolidine dithiocarbamate,PDTC)得到抑制。本实验的目的在于探讨抑制NF-κB的表达在宫颈癌HeLa细胞对射线诱导的细胞凋亡中的影响。方法:将HeLa细胞分为实验组加入PDTC(100μmol/L)及对照组不加PDTC。两组均给予直线加速器照射,剂量分别为0、2、4、6Gy,均作用2 h。用Western blot法来观察细胞核内NF-κB含量的变化,用TUNEL法来检测细胞的凋亡,用MTT法测细胞增殖。结果:辐射可以使HeLa细胞内NF-κB的表达增多,PDTC可以抑制由射线介导的NF-κB的增加。抑制了由射线介导的NF-κB的增加,不仅使得HeLa细胞的增殖受到明显的抑制(P<0.05)也使凋亡指数大大增加(P<0.001)。结论:在宫颈癌HeLa细胞中抑制NF-κB的表达可以增加由射线介导的细胞凋亡,增加了细胞对射线的反应。
Background and purpose： NF-κB is activated by tumor necrosis factor, some chemotherapeutic agents, and ionizing radiation. The activation of NF-κB could result in inhibition of apoptosis. NF-κB, regulated by PDTC （ pyrrolidine dithiocarbamate）, is a specific inhibitor of NF-κB. The purpose of our study was to explore the impact of inhibiting expression of NF-κB on radiation-induced apoptosis of uterine cervix cancer HeLa cells. Methods： Inhibition of NF-κB in He- La ceils was performed by treatment with 100 μmol/L PDTC for 2 hours. Cells were irradiated at 0, 2, 4 and 6 Gy with or without PDTC. The expression of NF-κB in nuclear was determined by western blot, and apoptosis was evaluated by using the TUNEL assay. Cell proliferation was evaluated by using MTT assay. Results： NF-κB activation was induced by radiation and inhibited by PDTC. Inhibition of radiation-induced NF-κB activation resulted in inhibiting cell proliferation （P 〈 0.05） and increased apoptosis index（ P 〈 0. 001） in HeLa cells. Conclusions： Inhibition of the expression of NF-κB increases radiation-induced apoptosis in uterine cervix cancer HeLa cells and enhances the cell response to radiation.
出处
《中国癌症杂志》
CAS
CSCD
2007年第7期521-523,共3页
China Oncology
基金
黑龙江省自然科学基金项目(No.D0320)
