摘要
制备大鼠肝抑素（ＨＣ）纯品，研究它对再生肝细胞和肝癌细胞体外增殖的影响。用ＤＥＡＥ－Ｃｅｌｌｕｌｏｓｅ阴离子交换层析法提取ＨＣ纯品，用液法检测ＨＣ样品的生物活性。结果：提取的ＨＣ纯品的ＳＤＳ电泳纯度达８９．５％， Ｍｒ １３．５ × １０３；该 ＨＣ纯品对大鼠再生肝细胞和人肝癌细胞体外增殖有明显抑制作用，抑制率分别为６３．１％（０．２ ｍｇ／Ｌ）和７７．７％（０．３ ｍｇ／Ｌ），但对人胃癌细胞增殖无抑制效应。结论：结果提示提取的ＨＣ是抑制再生肝细胞和肝癌细胞增殖的特异性因子。
PURPOSE To purify rat hepatic chalone, and study its effects in vitro on the proliferation of regenerating hepatocytes and hepatoma cells. METHODS The DEAE-cellulose anion exchange chromatography was used to purify hepatic chalone sample. The fluid scintillation was used to assay the biological activity of hepatic chalone sample. RESULTS The purified hepatic chalone is of 89.5 % purity and 13.5 x 103 molecular weight. This purified hepatic chalone showed remarkable inhibitory effect on proliferation of regenerating hepatocytes and human hepatoma cells in vitro with the inhibitory rate of 63.1 % (0.2 mg / L) and 77.7 % (0.3 mg / L) respectively, but did not show any inhibitory effect on the proliferation of human gastric carcinoma cells. CONCLUSIONS The purified hepatic chalone is a specific factor which can inhibit the proliferation of regenerating hepatocytes and hepatoma cells.
出处
《上海医科大学学报》
CSCD
1997年第1期7-10,共4页
Journal of Fudan University(Medical Science)
关键词
肝肿瘤
肝细胞
肝癌细胞
肝抑素
纯化
rat
hepatic chalone
purification
hepatocytes
hepatoma cells
