摘要
ＭＴＴ法是用于反映体外培养神经细胞活性的指标，但对检测细胞活性没有特异性。本实验结合神经元特异性烯醇化酶（ＮＳＥ）免疫细胞化学技术特异性强、敏感性高的优点与酶联免疫吸附试验酶促底物邻苯二胺（ｏｐｈｅｎｙｌｅｎｅｄｉａｍｉｎｅ，ＯＰＤ）呈色形成弥散的可溶性有色产物，通过检测有色溶液的ＯＤ值来比较体外培养过程中神经细胞的活性以及相应的数目，在建立此方法的同时并用此方法检测了人胚脊髓提取液对神经细胞活性的影响。结果证明：分子量小于１０ｋＤ的人胚脊髓提取液对体外培养的神经元存活以及生长有促进作用，用此方法比常用的ＭＴＴ方法检测更确切，并且还排除非神经元细胞的影响，对检测在各种条件（如神经生长因子、药物、缺血）作用下，比较神经细胞活性是一个很好的方法。
MTT is an important index to evaluate the viability of cultured neurons, but it has no specificity. NSE Immunocytochemistry technique has the strong specificity and high sensitivity for neuron and the DPD (O phenylendiamine) as a soluble substrate, can dissolve when it is used in ELISA techniques. We combined the NSE immuncytochemistry with ELISA methods to detect the viability of cultured neurons. With the establishment of this method, we tested the effect of human fetal spinal cord curde extract on the neurons. The results demonstrated that the extract fraction of <10kD had the promoting survival and growth effects on cultured neurons. Comparing to the MTT method, NSE ELISA is preciser and may be a good technique to evaluate the effect among NTFs and other factor on the neuron viabilities.
出处
《解剖学报》
CAS
CSCD
北大核心
1997年第1期32-35,共4页
Acta Anatomica Sinica
