摘要
采用硅胶柱色谱法对异叶败酱(Patrinia heterophylla Bunge.)化学成分进行了研究.通过理化性质及波谱学方法对分离得到的化合物进行结构鉴定,并运用SRB法对分离的部分化合物进行体外细胞毒性测试.从该植物中分离鉴定了10个化合物,分别为:软木三萜酮(Ⅰ),齐墩果酸(Ⅱ),熊果酸(Ⅲ),常春藤皂甙元(Ⅳ),海棠果醇(Ⅴ),齐墩果酸-3-O-α-L-吡喃阿拉伯糖苷(Ⅵ),β-香树精(Ⅶ),α-香树精(Ⅷ),β-谷甾醇(Ⅸ),胡萝卜苷(Ⅹ),除化合物Ⅱ之外,其余化合物都是首次从该植物中分离得到.SRB法结果表明:化合物Ⅱ、Ⅲ、Ⅳ和Ⅵ有较强的细胞毒性.
The chemical constituents from Patrinia heterophylla Bunge. are studied using silica gel column chromatography. Their structures are identified by spectral analysis and physical characteristics. Ten compounds are isolated and identified as friedelin （I）, oleanolic acid （Ⅱ）, ursolic acid （Ⅲ）, hederagenin（IV）, canophyllol（ V ）, oleanolic acid 3-O-α-L-arabinopyranoside（ Ⅵ）, β-amyrin（Ⅶ）, α- amyrin（Ⅷ）, β-sitosterol（IX）, daucosterol（ X ）. All of the compounds except compound Ⅱ are isolated for the first time from Patrinia heterophylla Bunge. The cytotoxicity of the compounds is tested by SRB assay, the results show that the compounds Ⅱ , Ⅲ, IV and Ⅵ have a certain cytotoxicity in vitro.
出处
《西北师范大学学报（自然科学版）》
CAS
2007年第3期62-65,共4页
Journal of Northwest Normal University(Natural Science)
基金
甘肃省教育厅科研基金资助项目(0601-27)
关键词
异叶败酱
化学成分
细胞毒性
Patrinia heterophylla Bunge.
chemical constituents
cytotoxicity
