摘要
用ＰＣＲ－ＰＡＧＥ方法，结合高灵敏的银染色作ＨＬＡ－ＤＱＡ１等位基因分型，研究ＤＱＡ１基因对类风湿关节炎（ＲＡ）的遗传易感性。选择无亲缘关系的广东籍汉族健康者１０６例和５０例ＲＡ患者。发现该方法测的６种ＨＬＡ－ＤＱＡ１等位基因中，ＲＡ组ＤＱＡ１＊０１０１（２７％，ＲＲ＝２．３３４，Ｐ＜０．００５，ＥＦ＝０．１５４）等位基因明显增高；而ＤＱＡ１＊０１０２（１％，ＲＲ＝０．０６８，Ｐ＜０．０１，ＰＦ＝０．５７７）明显下降；ＤＱＡ１的２种纯合子基因型（０１０１／０１０１和０３０１／０３０１）在ＲＡ组明显增高（Ｐ值分别小于０．０２５和０．００５）。上述结果显示：ＨＬＡ－ＤＱＡ１＊０１０１对ＲＡ有遗传易感作用，ＤＱＡ１＊０１０２等位基因有遗传抵抗作用；ＤＱＡ１基因型的检测对预测ＲＡ易感者和判断预后及疗效可能提供理论依据。
Had studied the genetic susceptibility of HLA DQA1 alleles to rheumatoid arthritis(RA) in Chinese Hans from Guangzhou (50 cases).DNA were extractied from various biological material by using phenlo/chloroform method.HLA DQA1 alleles were firstly amplified by PCR and then the products were fractionated by PAGE.The DNA bands were visualized by silver staining.Total 6 alleles of HLA DQA1 were identified.Increased allele frequencies of DQA1*0101(27%,RR=2.334,P<0 005,EF=0.154)and decreased allele frequencies of DQA1*0102(1%,RR=0.068,P<0 01,PF=0.577)were observed in case of RA.Increased the two genotyping of DQA1(DQA1*0101/0101;χ 2=5.215,P<0 025 and DQA1*0301/0301;χ 2=12.725,P<0 005)were observed.These results show that HLA DQA1*0101 contributes to the genetic susceptibility to RA while DQA1*0102 contribute to their genetic resistance in Guangdong Hans.Detection the genotyping of HLA DQA1 may be a useful marker to predict and avoid toxic side effect for the treatment of RA.Using PCR PAGE and silver dyeing technique,a new genotying method for HLA DQA1,which is simple,sensitive and precise,has been established and applied.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1997年第3期189-192,共4页
Chinese Journal of Immunology
