摘要
植物组织培养基的pH值一旦外植体植入就开始发生改变,直到达到某一平衡点。不同种的植物有其各自的pH平衡点。用6种植物(Ptilotus exaltatus Dees ex Lehm,Lechenaultia formosus R.Br.,Rosacanina L.,Melaleuca alternifolia(Maiden & E.Betche)Cheel,Anigozanthos flavidus DC.,Zieria cytisoides Sm.)培养在1/2 MS琼脂培养基中,pH平衡点的范围通常在2.8～4.3之间,pH的变化率也有差异。外植体植入到培养基中的前两周,植物体的重量会减少。在外植体植入前将培养基pH值调节至平衡点,这样避免了外植体植入后的pH变化。但是,这并没有克服外植体植入后前两周植物体重量的减少。矿物质的获取不受培养基pH值的影响。
It has been shown that the pH of agar medium changed steadily from the time when the explants were introduced until an equilibrium was reached. The equilibrium pH differed among plant species. Six species, Ptilotus exaltatus Dees ex Lehm, Lechenaultia formosus R.Br., Rosa cartina L., Melaleuca ahemifolia (Maiden & E.Betche) Cheel, Anigozantkos flamdus DC. and Zieria cytisoides Sm., cultured on 1/2 MS agar had pH equilibria in the range of 2.8 to 4.3. There were also differences in the rate of pH change. Explants lost weight over the first two weeks of culture. Equilibration of the pH of the medium before inserting the explants prevented the pH change but did not prevent the loss of plant weight. Mineral availability was not affected by the medium pH.
关键词
植物组织培养
培养基
PH值
pH change, Mineral availability, Plant tissue culture
