摘要
白杄(Picea meyeri Rehd.et Wils.)的成熟胚在4～6℃低温下保存1个月后,接种于改良LP+2mg/L 2,4-D+1mg/L 6-BA的培养基上,黑暗条件下培养1个月便可产生白色半透明的胚性愈伤组织。整体染色封片观察表明,胚性愈伤组织由很多很长的胚柄细胞及其顶端的胚细胞团组成,这种愈伤组织培养物称为胚性胚柄团。胚性胚柄团在MS+1mg/L 2,4-D+1mg/L KT的继代培养基上黑暗条件下可保持旺盛的增殖能力和分化潜力。当胚性胚柄团转到MS+5mg/L ABA+5mg/L AgNO_3的分化培养基上,1个月后可产生大量正常的体细胞胚。体细胞胚成熟以后转到含0.5％活性炭的无激素1/2MS基本培养基上约40d后可长出1.5～2.5cm的根,约60d后可长出真叶。光、ABA、蔗糖及AgNO_3浓度是影响体细胞胚发生的主要因素。
Translucent white embryogenic calli were produced from mature zygotic embryos of Picea meyeri Rehd. et Wils. which were stored at 4 to 6℃. for one month and then cultured on modified medium LP supplemented with 2 mg/L 2,4-D and 1 mg/L 6-BA in the dark for one month. It was shown from the whole callus-stained sections that the embryogenic callus consisted of a group of small meristematic cells at one end (embryonal end) and many long cells at the other (suspensor) which were named as embryogenic suspensor masses (ESM). ESM, when subcultured on medium MS supplemented with 1 mg/L 2,4-D and 1 mg/L KT in the dark, remained good reproductive and embryogenic potential. When ESM were transferred to MS medium supplemented with 5 mg/L ABA and 5 mg/L AgNO3 for one month, a lot of normal somatic embryos were produced. The mature somatic embryo developed root of 1.5 to 2.5 cm in length if cultured on 1/2 MS medium supplemented with 0.5% activated charcoal but without hormone for 40 d and leaves for 60 d. light,concentrations of ABA, sucrose and AgNO3 were the main factors of influencing somatic embryogenesis.
基金
863资助项目
