摘要
目的探讨胃癌淋巴结微转移的基因诊断方法,分析CK19 mRNA、MUC1 mRNA作为胃癌微转移检测分子标志物的可行性。方法应用逆转录-聚合酶链反应(RT-PCR)方法检测52例胃癌462枚常规病理阴性淋巴结中黏蛋白1(MUC1)mRNA、细胞角蛋白19(CK19)mRNA表达;对照组为4例胃良性病变患者的38枚淋巴结。结果胃良性病变患者38枚淋巴结中CK19 mRNA和MUC1 mRNA表达均为阴性;CK19、MUC1 mRNA在胃癌患者462枚常规病理阴性的淋巴结中223枚(48.3%)阳性表达,其中180枚(39.0%)淋巴结CK19 mRNA阳性表达,136枚(29.4%)MUC1 mRNA阳性表达,CK19 mRNA和MUC1 mRNA均为阳性者有125枚(27.0%)。不同组织类型淋巴结CK19、MUC1 mRNA阳性率差异无显著性(P>0.05),不同肿瘤浸润层次、原发肿瘤大小、肿瘤位置的阳性率差异有显著性(P<0.05)。结论MUC1、CK19基因均可作为RT-PCR法检测胃癌患者淋巴结微转移的分子标志物。
Objective To investigate the gene diagnosis of micrometastasis in lymph nodes in patients with stomach cancer and the feasibility of mucin 1 （ MUC1 ） mRNA and cytokeratin 19 （CK19） mRNA as molecular markers to detect micrometastasis of stomach cancer. Methods By using the reverse transcriptase-polymerase chain reaction （RT-PCR） technique, the expression of MUC1 mRNA and CK19 mRNA was detected in 462 lymph nodes （ taken from 52 patients with stomach cancer） with negative pathological findings. Another 38 lymph nodes （from 4 patients with gastric benign diseases） were examined as control. Results Neither CK19 nor MUC1 expression was observed in the 38 lymph nodes in the control group. Among the 462 lymph nodes from patients with stomach cancer, 223 lymph nodes （48.3%） were found positive expression of CK19 or MUC1 expression, including positive CK19 expression in 180 lymph nodes （39.0%） , positive MUC1 expression in 136 lymph nodes （29.4%） , and both in 125 lymph nodes （27.0%）. There was no significant difference in positive expression of CK19 and MUC1 mRNA in lymph nodes among different types of tissues （P 〉 0.05 ） , while significant differences were observed among different involvement depth, size, and location of tumors （P 〈0.05）. Conclusions The detection of MUC1 or CK19 mRNA expression using RT-PCR is helpful to diagnose the micrometastasis of stomach cancer.
出处
《中国微创外科杂志》
CSCD
2007年第7期697-700,共4页
Chinese Journal of Minimally Invasive Surgery
基金
新疆维吾尔自治区科技厅基金项目(XJEDU2004134):胃癌转移与微转移
