摘要
目的研究重组葡激酶(r-Sak)经不同途径溶栓治疗犬急性脑栓塞的疗效、并发症及对凝血纤溶系统的影响。方法成年毕格犬24条,随机分为对照组、r-Sak动脉组、r-Sak静脉组。用介入技术建立犬急性脑栓塞模型,栓塞后5h(静脉3h)行脑血管造影观察被栓塞的左颈内动脉通畅情况,继而经成功栓塞的左颈内动脉或股静脉于30min内注入r-Sak行溶栓治疗(r-Sak组:r-Sak10000u/kg;对照组:生理盐水10ml)。治疗后30、60和120min分别测定凝血指标并再行脑血管造影观察栓塞血管的再通情况,24h内对犬作行为学观察,24h后处死动物行病理检查。结果溶栓后2h对照组、r-Sak动脉组和r-Sak静脉组的血管再通率分别为0.0%、93.3%和37.5%,两治疗组与对照组比较差异有统计学意义(P<0.05);3组完全再通的比率分别为0%、60%和6.7%;血管再通率和完全再通率于动脉组明显高出静脉组(P<0.05)。r-Sak两组对凝血纤溶系统影响的比较无明显差异。24h内无严重并发症。结论重组葡激酶具有较强的血栓溶解作用,动脉途径给药比静脉法更能有效溶解脑内血栓。
Objective To evaluate the efficacy and complications in the treatment of dogs with acute cerebral infarction using recombinant staphylokinase（r-Sak） via different administration routes. Methods The model of left internal cerebral embolism was established with interventional technique in 24 beagle adult dogs which were randomly divided into control group, intraarterial group and intravenous group. Postembolization 5 hours（or 3 his in intravenous group）, a cerebral angiography was performded, a dosage of 10 000 u/kg r-sak was infused through left internal carotid artery or left femoral vein within 30 mins, and only 10 ml of saline was infused in control group. Angiography was repeated to observe the effects on recanalization and blood samples were collected to determine activated partial thromboplastin time, etc. at 30, 60 and 120 rains respectively after thrombolysis. The behavior of these canines was observed and all of the dogs were sacrificed after 24 hours for pathologic study. Results 2 hours after thrombolysis, recanalization rates of embolized cerebral vessels were 0%, 93.3% and 37.5% in control group, intraarterial group and intravenous group respectively, and the complete recanalization rates were 0%, 60% and 6.7%, respectively. There were significant differences between the two r-Sak groups and the control group （P 〈 0.05）, and the complete recanalization rate of intraarterial group was obviously higher than that of intravenous group （P 〈 0.05）. Influence on blood coagulation and fibrillolysis in two r-Sak groups showed no significant difference, and no serious complication occurred in 24 hours. Conclusion Thrombolysis using r-Sak is an effective treatment for canine models with acute cerebral infarction, and the thrombolysis effect of intraarterial method is much more than that of intravenous method.（J Intervent Radiol, 2007, 16： 397-400）
出处
《介入放射学杂志》
CSCD
2007年第6期397-400,共4页
Journal of Interventional Radiology
基金
江苏省医学重点人才项目(135-55)
关键词
重组葡激酶
溶栓
动脉或静脉途径
犬
脑栓塞
急性
Recombinant staphylokinase
Thrombolytic therapy,intraarterial or intravenous
Dogs
Cerebral infarction,acute
