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糖尿病肾病病人醛糖还原酶基因多态性相关研究

STUDY ON THE GENE POLYMORPHISIMS OF ALDOSE REDUCTASE IN TYPE 2 DIABETIC NEPHROPATHY
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摘要 目的探讨醛糖还原酶(AR)基因5′端(AC)n多态性与2型糖尿病肾病的关系。方法采用聚合酶链反应(PCR)、聚丙烯酰胺凝胶电泳及银染色技术分析了139例2型糖尿病病人(早期糖尿病肾病组61例,非糖尿病肾病组78例)和63例正常对照者AR基因5′端(AC)n的多态性。结果早期糖尿病肾病组Z-2等位基因频率高于非糖尿病肾病组和正常对照组(χ2=6.88、10.51,P<0.01);含Z-2等位基因的基因型频率也高于非糖尿病肾病组和正常对照组(χ2=4.95、8.15,P<0.05、0.01)。结论AR基因Z-2等位基因与青岛地区汉族2型糖尿病病人糖尿病肾病的发生发展有关。 Objective To investigate the association between （AC）n polymorphisms at 5-end of the aldose reductase gene and diabetic nephropathy of Type 2 diabetes mellitus. Methods The 5＇-ALR polymorphism in 139 patients with Type 2 diabetes （61 cases with nephropathy, 78 without nephropathy） and 63 healthy controls was identified in genomic DNA using polymerase chain reaction and PAGE gel electrophoresis together with silver-staining. Results The frequency of allele Z-2 was significantly higher in patients with diabetic nephropathy compared with that of without nephrophaty and controls （x^2= 6. 88,10. 51;P〈 0.01）. The genotypic frequency of allele Z- 2 was significantly higher in those with diabetic nephropathy compared with that of without nephrophaty and controls （x^2=4.95,8.15;P〈0.05,0.01）. Conclusion Allele Z-2 of aldose reductase may contribute to the pathogenesis of diabetic nephropathy with type 2 diabetes mellitus.
作者 钟丽娜 苗志敏 王忠超 李长贵
机构地区 青岛大学医学院附属医院内分泌科
出处 《青岛大学医学院学报》 CAS 2007年第4期308-310,共3页 Acta Academiae Medicinae Qingdao Universitatis
基金 山东省科技厅博士基金资助项目(02BS094)
关键词 糖尿病肾病 醛还原酶 基因型 基因频率 diabetic nephropathies aldose reductase geneotype gene frequency DN, DN
分类号 R587.2 [医药卫生—内分泌]
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参考文献9

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共引文献9

青岛大学医学院学报
2007年 第4期

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