摘要
目的通过研究高氧环境对未成熟大鼠视网膜线粒体超微结构的影响,为临床安全应用高氧,预防早产儿视网膜病变提供理论依据。方法将60只新生孕21d(足月为22～23d)SD大鼠随机分为空气组、高氧组,每组30只,每组又随机分为Ⅰ、Ⅱ、Ⅲ亚组各10只。空气组在空气中饲养;高氧组置于同一室的高浓度氧(>95%)箱中饲养。Ⅰ亚组出生当天取眼球,Ⅱ亚组出生7d取眼球,Ⅲ亚组出生14d取眼球。用电镜观察各亚组视网膜的结构,并对各亚组未成熟大鼠视网膜线粒体进行体视学研究。结果(1)高氧组在7d、14d视网膜线粒体出现显著肿胀的超微结构变化;(2)Ⅰ亚组间未成熟大鼠视网膜线粒体体积密度、表面积密度各参数均数间两两比较,差别无统计学意义(P>0.05);Ⅱ、Ⅲ亚组间未成熟大鼠视网膜线粒体各参数均数间两两比较,差异均有统计学意义(P<0.05)。结论高氧环境对未成熟大鼠视网膜线粒体超微结构有损伤作用。
Objective To study the effects of hyperxia on the retinal ultrastructure of nonage rats and provide the theory for safe application of hyperxia and prevention of retionopathy of prematurity. Methods Sixty new-born rats were randomly divided into air group and hyperxia group. Each group was divided into sub-group as 0 day, 7 days, 14 days after born. Rats of air group were raised in air and hyperxia group in trunk of 95% oxygen in the same room： Eyeballs of rats in every sub-group were removed at 0 day, 7 days, 14 days respectively. Retinal ultrastructure was observed by transmission electron microscope and retinal mitochondria were researched by methods of stereology. Results Retinal mitochondria were swelling obviously at 7 days, 14 days in hyperxia group;Volume density, surface density of retinal mitochondria had no obvious difference between sub-group of 0 day（P 〉 0.05 ） ,which were obviously different among sub-grottp of 7 days, 14 days（P〈0.05）. Conclusion Hyperxia is harm to retinal mitochondria of nonage rats.
出处
《眼科新进展》
CAS
2007年第7期489-491,共3页
Recent Advances in Ophthalmology
基金
广州市花都区医药卫生科技计划项目基金资助(编号:06HDWS002)~~
