摘要
用光敏生物素标记鸡新城疫病毒ｃＤＮＡ，制备核酸探针，经斑点杂交和碱性磷酸酶显色后，探针同该ｃＤＮＡ的ＰＣＲ产物、ＰＣＲ产物重组子和新城疫强弱毒株呈现阳性反应，与ＩＢＶ、ＩＬＴＶ、ＭＧ和正常尿囊液等均呈阴性杂交反应，田间病料的杂交试验也表明。
In this study,a cDNA probe of newcastle disease virus(NDV) labelled with photobiotin was prepared Using dot hybridization and the AV AP staining system,the probe showed strongly positive results when hybridized with a PCR product and its recombinant pSXⅣ/NDVF 0,but no hybridization signal was exhibited in other specimens such as IB,MG,ILT and normal allantoic fluid Three field samples suspected to be ND infection gave results which corresponded to the positive hybridization reactions The study showed that the photobiotin labelled NDV cDNA probe was a sensitive and specific method of NDV detection
基金
农业部博士基金
关键词
新城疫病毒
核酸探针
核酸杂交
newcastle disease virus
cDNA probe
nucleic acid hybridization
