摘要
目的研究沙利度胺和三氧化二砷( As2O3) 对急性非淋巴细胞白血病(ANLL)细胞分泌血管内皮生长因子( VEGF) 的影响, 并研究两者是否有协同作用。方法收集 23 例 ANLL 患者的骨髓液, 用 Ficoll 淋巴细胞分离液分离单个核细胞( MNC) , 于 37℃、5%二氧化碳 CO2 饱和湿度条件下于 RPMI1640 培养液中传代培养。取对数生长期细胞以 2×106/mL 的细胞浓度加入 24 孔培养板上, 沙利度胺以 75、150 和 300μg/mL 的浓度加入培养体系中, As2O3 以 12.5, 25 和 50μmoL/L 的浓度加入培养体系中, 并将 300μg/mL 的沙利度胺和50μmol/L 的 As2O3 联合加入培养体系中, 同时设空白对照。上述各组分别培养 48、72、96 和 120 h。用 ELISA法检测上清液 VEGF 浓度。结果沙利度胺和 As2O3 均可抑制 ANLL 细胞分泌 VEGF, 其抑制程度具有剂量 -时间依赖性。两药联用的抑制作用更加明显。结论白血病细胞能自分泌 VEGF; 沙利度胺和 As2O3 均能抑制ANLL 细胞分泌 VEGF; 两者联合应用起协同作用。
[Objective] To investigate the effect of thalidomide and As2O3 on the secretion of vascular endothelial growth factor （VEGF） of the cell of ANLL and study whether there is synergism between thalidomide and As2O3. [Methods] 23 patients with ANLL were analyzed. Heparinized marrow was taken, and putted into Ficoll-isopaque solution to purified MNC, cultured in the RPMI 1640 medium, placed in 5% CO2 saturation and 37℃ to prolifera- tion. The cells were chosen in logarithm growth phase, cultural solution was added to obtain the cellular density of 2×10^6/mL and then the cells were put into 24-well plates. Thalidomide of 75, 150 and 300p.g/mL, As2O3 of 12.5, 25 and 50 μmol/L, and thalidomide （300μg/mL） combined with As2O3 （25μmol/L） was added to substrate respectively, cultured for 48, 72, 96 and 120 hours, respectively. At the same time, a contrast group was set up. The concentration of VEGF in upper liquid was examined with ELISA method. [Result] Both thalidomide and As2O3 of different cancentrations can inhibit ANLL cells from secreting VEGF. The inhibition was dose-time dependent. Their combination shows more obvious inhibition. [Conclusion] Leukemia cell can secrete VEGF selfly; Both Thalidomide and As2O3 can inhibit the expression of VEGF in leukemic cells; Thalidomide can enhance the function of anti-leukemia of As2O3.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2007年第12期1418-1420,1426,共4页
China Journal of Modern Medicine
