摘要
基于核酸对富勒醇/镱Ⅲ体系共振光散射强度的增强作用,建立了一种新的测定核酸的分析方法.富勒醇的水溶液表现出弱的共振光散射性质,但是当三价镱离子存在时,它的共振光散射强度显著增强,形成了一种稳定的富勒醇/镱Ⅲ共振光散射体系.鱼精DNA的加入使得该体系的共振光散射强度进一步增强.在优化条件下,0 -20.0μg/mL浓度范围内,共振光散射强度的增强值与鱼精DNA的浓度呈线性关系,检出限可达13.3 ng/mL.该法用于合成样品的分析,结果令人满意.
A sensitive method for the determination of nucleic acids based on the enhancement of resonance light scattering （RLS） of fullerol/ytterbium（ Ⅲ） system has been developed. The fullerol showed a weak RLS spectrum in aqueous solution, but its intensity could be enhanced significantly in the presence of ytterbium（ Ⅲ） to form a stable RLS system. Moreover, the RLS signal of fullerol/ ytterbium（ Ⅲ ） system could be enhanced further by fish sperm DNA （fs DNA） and the enhanced RLS intensity was proportional to the concentration of fs DNA in the range of 0 - 20.0 μg/mL with a limit of detection of 13.3 ng/mL under the optimum conditions. The synthetic samples were analyzed with satisfactory results.
出处
《安徽师范大学学报（自然科学版）》
CAS
2007年第3期277-281,共5页
Journal of Anhui Normal University(Natural Science)
基金
Supported by National Natural Science Foundation of China (20475002)
the Science Foundation of Education Administration ofAnhui Province(2004KJ174)
关键词
富勒醇
镱
共振光散射
核酸
fullerol
Ytterbium
resonance light scattering（RLS）
nucleic acid
