摘要
运用单抗技术我们制备了六个单抗,其中四个分别识别在果蝇卵子发生不同时间和空间表达的抗原。B2抗原最早出现,主要由包囊细胞和营养细胞合成。该抗原通过运输进入并定位在卵母细胞后区。F9抗原随后出现在卵细胞的后区。继而E8抗原表达并定位在卵细胞膜上。C3抗原表达最晚。主要分布在成熟卵的前后两端外侧的卵黄膜间隙中。这些抗原所具有的独特表达和分布图式提示它们在卵子发生中可能具有一定的独特功能。
By use of hybridoma technique, we have prepared 6 monoclonal antibodies. As shown by antibody labeling of whole mount ovary, four of them recognized, respectively, the antigens which were spatial-temporally expressed during oogen-esis. The B 2 antigens appeared very early in the germarium and were expressed mainly by cystocyte and nurse cells.Later, they were all transported and localized in the posterior of oocyte, F 9 antigens followed and were also transported and lo-
calized in the posterior of oocyte in the stage 7-8. Then, E 8 antigens appeared and mainly localized on the membrane of oocyte in the stage 9-10. C 3 antigens were expressed much later, at about stage 14.They localized like two caps in the perivitelline fluid at both ends of matured egg. Such specific expression and distribution pattern of all these antigens suggest their possible roles during ooge-nesis.
出处
《实验生物学报》
CSCD
1997年第1期91-97,共7页
Acta Biologiae Experimentalis Sinica
基金
国家自然科学基金会和中国科学院上海生命科学联合开放研究实验室资助
关键词
果蝇
卵细胞发生
蛋白
Drosophila. Oogenesis. Monoclonal antibody
