摘要
目的探讨12-LOX选择性抑制剂baicalein的药物毒性,为baicalein的临床应用提供参考。方法体外原代培养兔角膜上皮细胞,加入30μmol/L baicalein继续培养2、4、6d,MTT法测定细胞抑制率。制作兔角膜上皮缺损模型,同浓度baicalein制成滴眼药局部应用,观察其对在体兔角膜上皮增生和移行的影响。结果30μmol/L baicalein对体外原代培养兔角膜上皮细胞作用2d的细胞抑制率为2.6%,作用4d、6d对细胞生长没有明显的抑制作用。缺损闭合实验显示此浓度baicalein对在体兔角膜上皮细胞的增生和移行无任何影响。结论30μmol/L baicalein安全,对兔角膜上皮细胞的生长无影响。
Objective It has been showed that baicalein, a selective inhibitor of 12-LOX, have an inhibition on angiogenesis and suppress corneal neovascularization in rabbits effectively. However, its safety to ocular tissue has not been reported yet. This study tried to investigate the toxicity of baicalein to corneal epithelial cells of rabbit and offer a reference of clinical application of baicalein. Methods Primary cultured corneal epithelial cells of rabbit were exposed to 30 μmol/L baicalein for 2,4,6 days. The inhibition rates of corneal epithelial cells were determined by MTT assay. Rabbit corneal epithelial cells defect models were produced in vivo. Eight models were divided into experimental group and control group. In the experiment group,baiealein in 1% dimethyl sulfoxide （DMSO） and in 10% Tween 20 buffer solution （ the final concentration of baicalein was 30 μmol/L ） was topically applied 4 times daily for 10 days in the right eyes of rabbit,and 1% DMSO and 10% Tween 20 buffer solution only were applied topically in the left eyes in the control group. The defect area of rabbit corneal epithelial cells was assessed at 6,12,18 and 24 hours by Image Pro Plus V4.5 software. Results The inhibiting rate of baicalein on cultured corneal epithelium cells was 2. 6% at 2 days,and no significant suppressing effects were found at 4 and 6 days. No statistically significant differences were seen between experimental group and control group at 2,4,6 days （P 〉 0.05）. The defect area of corneal epithelial cells at 0,6, 12 and 18 hours had not statistically significant differences between experimental and control groups. The defect area in the center and periphery of cornea was gradually reduced with time lapse. Conclusion Baicalein at 30 μmol/L is safe to ocular surface tissue during the topical utilization. Baicalein has no significant influence on the proliferation and migration of corneal epithelial cells.
出处
《眼科研究》
CSCD
北大核心
2007年第7期537-539,共3页
Chinese Ophthalmic Research
