摘要
目的：探讨纤维连接蛋白（Ｆｎ）在创伤后脓毒症多器官损害（ＭＯＤ）中的作用与机制，为临床应用Ｆｎ制剂防治创伤后ＭＯＤ提供理论依据。方法：在创伤后３小时输注Ｆｎ治疗，观察创伤后脓毒症小鼠和用Ｆｎ治疗后小鼠的血浆Ｆｎ、肿瘤坏死因子α（ＴＮＦα）水平和肝、肾、小肠的组织病理变化。结果：创伤后３小时血浆Ｆｎ水平（９２．１７±１０．１２ｍｇ／Ｌ）较正常对照（１２８．１７±１０．２５ｍｇ／Ｌ）显著降低（Ｐ＜０．０５）；脓毒症小鼠肝、肾、小肠均出现明显病理损害，肝小叶Ｆｎ聚集沉着；Ｆｎ治疗组血浆Ｆｎ水平（２１６．００±２４．５０ｍｇ／Ｌ）明显高于脓毒症组（１９９．４０±１６．９０ｍｇ／Ｌ），Ｐ＜０．０５；而其血浆ＴＮＦα水平（０．７７±０．０３μｇ／Ｌ）低于脓毒症组水平（０．８０±０．０４μｇ／Ｌ），肝、肾、小肠组织病理损害明显减轻，肝组织Ｆｎ与正常相似。结论：Ｆｎ治疗能有效防止创伤后脓毒症ＭＯＤ发生，可能与单核巨噬细胞廓清功能改善和ＴＮＦα释放减少的双重效应有关。
Objective:To investigate the role and mechanism of fibronectin (Fn) in development of multiple organ damage (MOD) in posttrauma septic mice,further providing evidence to support Fn treatment following trauma.Methods:The mice were treated with Fn or normal saline at 3 hours posttrauma.Changes in plasma Fn,TNFα levels and histologic damage in liver,kidney and small intestine were observed in both control and Fntreated mice.Results:Plasma Fn levels decreased markedly at 3 hours posttrauma compared with the normal controls (92 17±10 12 vs. 128 17±10 25mg/L, P <0 05).The histologic damage in liver,kidney and small intestine was evident together with remarkable Fn deposit in liver in posttrauma septic mice.Following Fn treatment,however,plasma Fn levels increased significantly (216 00±24 50 vs. 199 40±16 90mg/L, P <0 05) and TNFα levels decreased (0 77±0 03 vs. 0 80±0 04μg/L).Moreover,the histologic damage were abated markedly.Conclusions:Fn can markedly protect mice from developing sepsis and multiple organ damage following trauma,and it might be associated with both improvement of monocytemacrophage clearance function and reduction of TNFα release.
出处
《中国危重病急救医学》
CAS
CSCD
1997年第5期257-258,共2页
Chinese Critical Care Medicine
基金
硕士研究生课题
