摘要
目的：了解内毒素（ＬＰＳ）、肿瘤坏死因子（ＴＮＦ）和白细胞介素１（ＩＬ１）对实验性内毒素休克时肺损伤的保护作用。方法：实验分３组：Ⅰ组为假灌流组，Ⅱ组、Ⅲ组大鼠在内毒素休克早期开始分别给予活性炭（Ⅱ组）、大孔树脂ＡｍｂｅｒｌｉｔｅＸＡＤ７（Ⅲ组）血液灌流，结果与Ⅰ组作比较。结果：Ⅱ组ＬＰＳ、ＩＬ１水平明显降低，而Ⅲ组ＴＮＦ水平明显降低。Ⅱ组、Ⅲ组外周血白细胞数分别为（４．０±０．４）×１０９／Ｌ和（３．９±０．４）×１０９／Ｌ，均明显高于Ⅰ组〔（３．１±０．２）×１０９／Ｌ〕，Ｐ均＜０．０１；支气管肺泡灌洗液（ＢＡＬＦ）中的白细胞数〔（８．５±０．１）×１０９／Ｌ，（８．９±０．１）×１０９／Ｌ〕、肺系数（０．６４±０．０６，０．６６±０．０６）以及肺组织浸润的中性分叶核粒细胞数（２９．６±７．９／ＨＰ，３４．５±４．１／ＨＰ）均明显低于Ⅰ组〔（１２．３±１．８）×１０９／Ｌ，０．７５±０．０７，４８．６±６．６／ＨＰ〕，Ｐ均＜０．０１或＜０．０５。Ⅱ组、Ⅲ组的肺组织学改变亦较Ⅰ组明显减轻。结论：清除血液中致病介质可能对内毒素休克时的肺损伤产生保护作用。
Objective:In order to investigate the effect of lipopolysaccharide,tumor necrosis factor and interleukin1 on experimental endotoxic shockinduced lung damage.Methods:Hemoperfusion with activated charcoal(group Ⅱ) or Amberlite XAD7(group Ⅲ)were performed in the early stage of experimental model of endotoxic shock in rats.Results:The blood WBC counts of two treated groups were (4 0±0 4)×10 9/L,(3 9±0 4)×10 9/L respectively,which were significantly higher than that in sham group group Ⅰ,(3 1±0 2)×10 9/L,all P <0 01 .Both the WBC counts in bronchoalveolar lavage fluid (8 5±0 1)×10 9/L,(8 9±0 1)×10 9/L and pulmonary coeffecient (0 64±0 06,0 66±0 06) were significantly lower than that in two treated groups (all P <0 01)when compared with group Ⅰ.The pathological lessions were ameliorated and less infiltrating neutrophils were noted in two treated groups(29 6±7 9/HP,34 5±4 1/HP vs 48 6±6 6/HP,all P <0 05.Conclusions:It is suggested that removal of deleterious mediators from circulation by hemoperfusion may be of protective effect on endotoxic shockinduced lung damage.
出处
《中国危重病急救医学》
CSCD
1997年第4期193-195,共3页
Chinese Critical Care Medicine
基金
国家自然科学基金
广东省科学基金
