摘要
目的观察不同月龄自发性高血压大鼠(SHR)肾脏血管紧张素转换酶2(ACE2) mRNA转录及其蛋白表达,初步探讨ACE2在高血压发生、发展过程中的可能作用。方法雄性SHR1月龄组(S1)、2月龄组(S2)、3月龄组(S3)、6月龄组(S6)和9月龄组(S9)共5组,每组各6只,各组均有相应月龄匹配的Wistar-Kyoto(WKY)大鼠作对照。采用RBP-Ⅰ型大鼠血压心率测定仪测量大鼠尾动脉收缩压(SBP);逆转录聚合酶链式反应(RT-PCR)法检测肾脏ACE2 mRNA的转录水平;免疫组化染色结合计算机图像分析方法测定肾脏ACE2蛋白的表达水平。结果1)SHR的SBP随着月龄的增加而上升,6月龄后趋于稳定。2)SHR和WKY肾脏ACE2蛋白和 mRNA水平均随着月份的增加而增加,3月龄时达高峰,6月龄后趋于稳定;且SHR肾脏ACE2蛋白和 mRNA水平均低于同龄的WKY。S1肾脏髓质内侧部ACE2免疫染色阳性面积百分比较皮质和髓质外侧部高,与1月后的分布相反。结论1)SHR肾脏ACE2 mRNA和蛋白的表达水平比WKY大鼠低。2)大鼠肾脏ACE2 mRNA和蛋白的表达具有时间和部位分布上的差异。
Objective To investigate the expression of angiotensin-converting enzyme 2（ACE2） protein and mRNA in kidney of different age spontaneously hypertensive rats{ SHR） and the role of ACE2 in the development of hypertension. Methods Male SHR（n=30） were allocated to 5 groups by age： 1 lone-month-old group（S~ ） ; 2）two-month-old group（S~ ） ; 3）three-month-old group（Ss ） ; 4）six-month-old group（S6 ） ; 5）nine-month-old group （S9）. Sex and age matched Wistar-Kyoto normotensive rats （WKY）served as controls. The expression of ACE2 mRNA in kidney was determined by reverse transcription polymerase chain reaction（ RT-PCR）. The expression of ACE2 protein in kidney was assessed by immunohistochemistry and computer image analysis. Results 1 ）An age-dependent increase of SBP was observed in SHR before six months（ P〈0. 05）, however, after the sixth month SBP remained stable（ P〉0.051. 2 ）An age-dependent increase of the ACE2 protein and mRNA levels in kidney was observed in both SHR and WKY（P〈0. 05）, which reached the peak in the third month（P〈 0. 05）and remained stable after the sixth month（P〉0. 05）. The ACE2 protein and mRNA levels in kidney were lower in SHR than those in the matched WKY at every corresponding time point （P〈 0. 051. 3 ）The ACE2 histehemieally stained area was higher in the inner medulla region than that in the cortex and outer medulla in one- month-old SHR and WKY （P〈0. 051, which was different from the distribution of other groups, the significance of which needs further elucidated. Conclusion The expression of ACE2 mRNA and protein in kidney of SHR is decreased compared with WKY. With location and time difference in the expression of ACE2 mRNA and protein was manifested in kidney.
出处
《中华高血压杂志》
CAS
CSCD
北大核心
2007年第6期473-476,共4页
Chinese Journal of Hypertension
关键词
血管紧张素转换酶2
自发性高血压
大鼠
肾脏
高血压
Angiotensin-converting enzyme 2
Spontaneously hypertensive
Rat
Kidney
Hypertension
