摘要
蒲黄经热水提取,脱除蛋白质、乙醇沉淀,Sephadex G50柱层析分离,DEAE-Sephadex A25和DEAE-Cellulose离子交换柱层析分离得3种白色粉末状多糖TAA、TAB、TAC。经凝胶柱层析、醋酸纤维素薄膜电泳证明,三者为均一体。3种多糖经酸全水解、纸层析和气相色谱分析,确定了糖基的组成及摩尔组成比。用核磁共振、甲基化分析、NaIO_4氧化等方法对多糖结构进行了初步探讨。
Three polysaccharides TAA, TAB, TAC have been isolated, purified from the pollen of Ty-pha angustifolia L. by hot water extraction, alcohol precipitation, sevage method, Gel filtration (Sephadex G50), ion-exchange chromatography on DEAE Sephadex A25 and DEAE-cellulose.The structure determination was carried out by paper chromatography of the hydrolysis products, GLC analysis of the alditol acetates, methylation analysis, 1H NMR and 13C NMR spectra. TAA is mainly composed of a-L-arabinofuranose, β-D-galactose and a-D-galacturonic acid. TAC contains the backbone mainly composed of (1→5)-linked-.L-arabinosyl residue.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1990年第12期1362-1366,共5页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
关键词
蒲黄
花粉
多糖
烷基化分析
Pollen, Polysaccharide, Alkylation analysis
