摘要
以氨丙基多孔硅球作载体,以戊二醛作交联剂制备的固定化嗜热菌蛋白酶催化合成Aspartame前体,通过酸化脱盐、氢化等反应得到二肽甜味剂Aspartame。研究了反应条件对合成Aspartame前体产率的影响,考察了固定化酶的重复使用性能。结果表明,固定化酶在水相中能较好的催化合成Aspartame前体,且具有良好的重复使用性能。
In this paper, immobilized thermolysin was prepared by using aminopropyltriethoxysilane treated porous silica beads as carrier, and glutaraldehyde as crosslinking agent. The precursor of aspar-tame was synthesized in aqueous medium in good yield by the catalysis of the immobilized thermolysin , and aspartame was then obtained by the catalytic hydrogenolysis of the precursor. In the immobilized thermolysin-catalysed coupling reaction, the factors which affect the yield such as reaction time, temperature, medium pH, amount of the immobilized enzyme, substrate concentration and substrate molar ratio as well as concentration of calcium acetate were studied. Reuse of the immobilized thermolysin was also investigated.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1990年第6期643-646,共4页
Chemical Journal of Chinese Universities
关键词
嗜热菌蛋白酶
甜味剂
催化合成
Thermolysin, Immobilized enzyme, Sweetener, Catalytic synthesis
