摘要
目的:制备犬脱钙骨基质(demineralized bone matrix,DBM),研究DBM作为骨组织工程支架材料的可行性。方法:杂种犬的股骨行脱脂、脱钙、脱非胶原蛋白、冷冻干燥、灭菌制成DBM,与骨髓间充质干细胞(mesenchymal stemcells,MSCs)复合,应用倒置相差显微镜、扫描电镜观察脱钙骨基质的结构及细胞在材料表面的生长情况。结果:犬脱钙骨基质具有三维立体网孔结构,平均孔隙直径为(254.39±88.71)μm,孔隙率约为70%。与MSCs复合培养后,细胞黏附其上,上架率高,生长状态良好,增殖迅速,并分泌大量细胞外基质。结论:DBM具有良好的生物相容性。
PURPOSE： To manufacture demineralized bone matrix（DBM） of mongrel and to explore the feasibility of DBM as scaffold of bone tissue engineering. METHODS： Thigh bones of mongrel were degreased, demineralized, deproteined, freezed, dried and sterilized to form DBM. Mesenchymal stem cells（MSCs） were seeded onto the scaffold and their growth were examined by inverted phase contrast microscope and scanning electron microscope. RESULTS： DBM had a three-dimensional mesh structure.The mean pore diameter of DBM was （254.39±88.71）μm and the pore rate was about 70%.MSCs could adhere to the surface and inner walls of DBM, proliferated well and secreted a large amount of extracellular matrix. CONCLUSION： DBM has satisfactory biocompatibility. Supported by National Natural Science Foundation of China （Grant No.30471899）.
出处
《上海口腔医学》
CAS
CSCD
2007年第3期255-258,共4页
Shanghai Journal of Stomatology
基金
国家自然科学基金(30471899)
关键词
骨髓间充质干细胞
脱钙骨基质
组织工程
Mesenehymal stem eells
Demineralized bone matrix
Tissue engineering
