摘要
为了得到纯的SARS-CoV E蛋白以进一步研究其功能,通过Touch-down PCR程序,从SARS-CoV WHU cDNA(pMD18-T载体)文库中扩增了E基因,构建了重组质粒pGEX-E,并在Ecoli DH5α中进行了原核表达。SDS/PAGE及Western blot结果表明SARS病毒E蛋白的分子量约5 kDa,较预测的略小,并进一步分析了这种现象产生的可能原因。
To obtain the pure sample of SARS-CoV E protein to further study its function, the gene fragment encoding E protein was amplified by touch-down PCR, the recombinant plasmid pGEX-E was further constructed, and the E protein was then expressed in E coli DH5α. The results of SDS/PAGE and Western blot suggested that the E protein had about 5 kDa molecular weight that was smaller than the estimated molecular mass and the possible causes were further analyzed.
出处
《重庆三峡学院学报》
2007年第3期106-107,共2页
Journal of Chongqing Three Gorges University
基金
国家科技部973计划项目(2003CB514120)资助。
关键词
SARS病毒
克隆
表达
SARS-associated coronavirus （SARS-CoV）
Cloning
Expression
