摘要
对张家口分离的马铃薯S病毒（Potato virus S,PVS-Hebei）的CP基因进行了克隆和序列分析。以提纯的马铃薯植物总RNA为模板,应用RT-PCR技术扩增目的基因,PCR扩增产物克隆到质粒pMD18-T上,并进行了序列分析。结果表明,克隆的cDNA片段由885个核苷酸组成,编码294个氨基酸组成的33 kD蛋白,与PVS外壳蛋白的大小一致,与国际基因库登记的几个株系相比,结果表明,PVS-Hebei与它们之间的核苷酸序列同源性为82.4%-95.6%,氨基酸序列的同源性为93.9%-98.0%,进化树分析表明,PVS明显存在2个组（组Ⅰ和组Ⅱ）,PVS-Hebei归类为Ⅰ组,与来自组Ⅰ的欧洲普通株系（PVS-Uk）的亲缘性要比来自组Ⅱ北美的Andean株系（PVS-A）株系的亲缘性要近。
A isolate of potato virus S （PVS-Hebei） was obtained from potato plants cultivated in Zhangjiakou of Hebei province and the coat protein （CP） gene was coined and sequenced. The CP gene was amplified from total extracted potato RNA by reverese transcription-polymerase chain reaction （RT-PCR）. The specific PCR fragment then cloned in to the pMD18-T vector and sequenced. The result showed that the cloned segment was 714 bp and encodes 294 amino acid residues with relative molecular weight of 33 kD which is the length of the PVS CP, Comparison of the nucleotide sequence between the CP gene and those of several PVS isolates showed that the homology of are 82.4 % - 95.6 %, and the homology of the putative amino acid sequence are 93.9 % - 98.0 %, respectively. The evolutionary distances analysis suggested that PVS isolates was divided into two groups, PVS-Hebei was assigned into group Ⅰ and that it was close to the ordinary European isolates of ordinary strain of PVS （PVS-Uk） but distant to the original Andean strain of PVS （PVS- A）.
出处
《华北农学报》
CSCD
北大核心
2007年第3期39-42,共4页
Acta Agriculturae Boreali-Sinica
基金
河北省科技攻关项目(04225505)
