摘要
目的:观察丹参和枸杞对中波紫外线(UVB)损伤角质形成细胞的保护作用及作用机制。方法:用丹参和枸杞对HaCaT细胞进行预处理24小时,采用20、40、60mJ/cm2剂量的UVB照射细胞,以MTT法检测细胞生存率,以酶联免疫吸附实验检测细胞上清液中TNF-α及IL-1β的分泌量。结果:角质形成细胞损伤程度与UVB照射剂量有关,丹参和枸杞均可提高UVB照射后角质形成细胞的生存率,丹参可抑制角质形成细胞释放TNF-α和IL-1β;枸杞只抑制TNF-α的释放,而对IL-1β无明显抑制作用。结论:UVB对角质形成细胞有损伤作用,且与剂量相关;丹参、枸杞可以通过降低UVB引起的炎症因子TNF-α和(或)IL-1β的分泌从而减轻紫外线辐射引起的皮肤损伤。
Objective： To investigate the photo-protective effect of the traditional Chinese medicine （Salvia Miltiorrhiza and Fructus Lycii） on human keratinocytes from ultraviolet B（UVB） radiation. Methods： After pretreated with Salvia Miltiorrhiza or Fructus Lycii for 24 hours, HaCaT Cells were irradiated with different dosages of UVB. The cell viability was detected by MTT method, The supernatant was collected at 24 hours post irradiation to measure the secretion of cytokine TNF-α,and IL-1β by ELISA. Results; The cell viability was inverse correlating with the irradiated dosage. Both Salvia Miltiorrhiza and Fructus Lycii could boost the cell viability. Salvia Miltiorrhiza could reduce the secretion of TNF-α and IL-1β, Fructus Lycii could only reduce the secretion of TNF-α. Conclusions： The damage of UVB irradiation on human keratinocyte was dose-dependent. Salvia Miltiorrhiza and Fructus Lycij have photo-protective potency, Inhibition of TNF-α or IL-1β secretion may be one of the mechanisms for photo-protection.
出处
《江西中医学院学报》
2007年第3期61-63,共3页
Journal of Jiangxi College of Traditional Chinese Medicine
关键词
中波紫外线
角质形成细胞:光保护
中药
Ultraviolet B
Keratinocytes
Photo-Protection
Traditional Chinese Medicine
