摘要
目的通过对肺结核患者外周血树突细胞(dendriticcell,DC)亚群数量的观察,了解DC亚群在肺结核病程中的变化情况,探讨DC亚群在肺结核发病机制中的作用及临床意义。方法采用流式细胞术的三色分析法检测70例肺结核患者外周血的DC1及DC2亚群。结果肺结核活动期患者外周血DC1/PBMC(外周血单个核细胞)、DC2/PBMC及DC1、DC2的绝对数均明显低于健康者(P<0.05),DC1/DC2值明显高于健康者(P<0.05);肺结核非活动期患者与健康者外周血DC1/PBMC、DC2/PBMC及DC1、DC2的绝对数比较均无显著性差异(P>0.05),与健康者比较DC1/DC2值无显著性差异(P>0.05);肺结核活动期患者外周血DC的绝对数明显低于非活动期(P<0.05)。结论肺结核患者外周血DC1和DC2数均明显降低。提示外周血树突细胞亚群(DC1、DC2)的检测可了解肺结核患者的免疫功能状态及反映病情变化,也说明DC在结核病免疫损伤的发生发展中发挥一定作用。
Objective To observe the changes of peripheral blood dendritic cell （DC） subgroup in amount in pulmonary tuberculosis patients. Methods DC1 and DC2 subgroups were detected in peripheral blood of 70 pulmonary tuberculosis patients by tricolor analytic method of flow cytometry. Results In active stage of pulmonary tuberculosis, DC1/PBMC, DC2/PBMC and the absolute quantity of DC1, DC2 in peripheral blood were significantly lower than healthy subjects （P 〈0.05 ） and DC1/DC2 ratio was significantly higher （P 〈0.05）. In inactive stage of pulmonary tuberculosis, DC1/PBMC, DC2/PBMC and the absolute quantity of DC1, DC2, DC1/DC2 ratio in peripheral blood showed no significant difference with that of healthy subjects （P 〉 0.05） ; The absolute quantity of DC2 in active stage of pulmonary tuberculosis was obviously lower than that in inactive stage （ P 〈 0.05 ）. Conclusion The percentage and the absolute quantity of DC1 and DC2 subgroups in peripheral blood in active stage of pulmonary tuberculosis are lower than the healthy subjects, suggesting DC can reflect the immune function of pulmonary tuberculosis patients, the patients＇ condition and illuminate the immunopathogenesis of pulmonary tuberculosis.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第13期1298-1300,共3页
Journal of Third Military Medical University
基金
合肥医学院青年科研启动基金(2006
F-183)~~
关键词
肺结核
树突细胞亚群
流式细胞术
pulmonary tuberculosis
dendritic cell subgroup
flow cytometry
