摘要
以不同剂量的伊氏锥虫排泄分泌抗原（ＥＳＡ）对小鼠进行常规免疫，用同株伊氏锥虫攻击，观察并记录小鼠死亡时间、死亡只数，比较免疫后血清抗体水平。结果表明：除对照组和５μｇ组未产生特异性抗体外，其余各组均产生较高滴度的抗体，其中２００μｇ组为１∶６４００，１００μｇ组为１∶３２００。ＥＳＡ５０，１００和２００μｇ免疫组可对小鼠产生有效的保护，小鼠存活时间较对照组延长２～３倍。
After immunized with excretion secretion antigens (ESA) of Trypanosoma evansi in different quantity, mice were challenged with Nanjing buffalo Trypanosoma evansi 1×10 2/mice intraperitoneally. The times and numbers of dead mice were recorded and serum antibody titers were detected. The data were compared among several groups. The results were shown as follows: specific antibodies were found in most vaccinated mice except the control and 5 μg groups. Antibody titers in the mice vaccinated 200 and 100 μg doses were 1∶6 400 and 1 ∶3 200, respectively. ESA at doses of 50, 100, 200 μg provided effective protection to mice.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
1997年第2期87-89,共3页
Journal of Nanjing Agricultural University
关键词
伊氏锥虫
排泄分泌抗原
抗原
免疫原性
Trypanosoma evansi
excretion secretion antigen
mice
immunogenicity
