摘要
目的建立颅咽管瘤(CP)动物模型,检测移植瘤的微循环结构和瘤细胞分裂、增殖能力。方法收集人脑颅咽管瘤组织,异种异位移植到BALB/C裸小鼠皮下,建立移植瘤。采用免疫组化法以cytokeratin(CK)鉴定肿瘤表型,CD34标记血管内皮细胞。微染色体维持蛋白6(MCM6)检测瘤细胞增殖活性。结果成功建立了10只动物模型。移植瘤组织自身形成微血管,瘤细胞保持了分裂、增殖活性。微血管密度(MVD)、MCM6二者呈正相关(r=0.410,P<0.05)。釉质上皮型(AE)与鳞形乳头瘤型(SP)移植瘤MVD、MCM6比较差异无显著性意义(P>0.05)。结论采用异种异位移植方法可在裸小鼠皮下初步建立人脑颅咽管瘤动物模型。移植瘤细胞可建立新的微循环结构,具备一定的分裂、增殖能力和血管形成活性。
Objective To establish the animal model of craniopluryngioma （CP） and detect the microcirculation and cell division growth activity of transplanted tumor. Methods Human craniopharyngioma tissue was heterotopically transplanted into BALB/C nude mouse subcutaneously. cytokeratin （CK） was detected by immunohistochemistry （SABC） to determine the phenotype of transplanted tumor. CD34 marked vascular endothelial cells, and minichromosome maintenance protein 6 （MCM6） expression indicated the proliferative ability of tumor cells. Results Ten animal models were successfully established. Total success rate of transplantation was 39.58%, that of adamantine epithelioma （AE） was 58.33%, and that ofsquamous papillary tumor （SP） was 20.83%. Transplantated tumors reconstructed capillary vessels, which stretched into the center of tumor essence, and tumor cells maintained division growth activity. Microvessel density （MVD） and MCM6 had a positive correlation in the transplantated masses （r=0.410, P〈0.05）, but MVD and MCM6 were not significantly different between AE and SP （P〉0.05）. Conclusion Animal model of human craniopharyngioma can be established in nude mouse subcutaneously by heterotopic transplantation. The tansplantated tumors keep the bionomics of craniopharyngioma and possess a new blood circulation and cell proliferative ability.
出处
《中华神经医学杂志》
CAS
CSCD
2007年第6期585-588,共4页
Chinese Journal of Neuromedicine
基金
中国博士后基金(20060391021)
四川省科技厅基础研究项目(04JY029-002-13)
