摘要
椰子幼花序在改良的MS附加高浓度的NAA与2，4-D的培养基中产生愈伤组织，在逐渐降低NAA和2，4-D浓度的过程中，愈伤组织产生结构紧密的结节组织。结节组织内有输导组织、分生细胞团、根原基及不同发育阶段的胚状体，有些进而发育成正常的根和绿色芽状物。同时，某些幼花枝的基部直接长出绿芽，经继续培养形成具有正常很、叶结构的完整植株，但茎尖无顶端分生组织，无叶原基，有的发育成花枝，甚至在顶部长出几朵发育不正常的小花。
Callus was induced in the modified MS hasal medium with high concentration of NAA and 2,4-D from young inflorescence of coconut (Cocos nucifera L. ). During the callus subculture with gradually reduced concentration of NAA and 2,4-D,it developed into compact nodular tissue. There were conducting tissue,meristematic cell clumps,root primordia and embryoids at different developing stage in the nodular tissue. The normal roots and green buds were developed subsequently from some of the nodular tissue. At the same time,some green buds grew out directly from the base of the young flower branches, and finally became the plantlets with normal roots and leaves,but without apical meristem and leaf primordia. Moreover,some of the stem apex developed into flower branches and even showed several small abnormal flowers.
出处
《武汉植物学研究》
CSCD
1997年第1期39-42,共4页
Journal of Wuhan Botanical Research
基金
广东省科委资助
关键词
椰子
幼花序
离体培养
器官发生
Young inflorescence of coconut, In vitro culture, Organogenesis
