摘要
目的观察体外缺氧条件下骨肉瘤SaOS-2生物学特性的改变。方法建立骨肉瘤细胞体外缺氧培养模型;流式细胞仪分析细胞周期改变;划痕损伤法和Boyden侵袭小室检测细胞体外侵袭迁移能力的变化;半定量逆转录-聚合酶链反应(RT-PCR)和免疫组织化学(SP法)观察缺氧培养不同时相(8、16、24 h)血管内皮生长因子(VEGF)的表达。结果缺氧培养16 h内,细胞周期表现为G_1期阻滞。24 h后,细胞周期恢复常氧状态。缺氧培养24 h后,骨肉瘤细胞体外迁移和侵袭能力明显增强。缺氧处理后,VEGF mRNA以及蛋白的表达在缺氧条件下均显著增强。结论骨肉瘤细胞存在缺氧耐受机制,通过一系列生物学特性的改变来应对缺氧带来的不利。
Objective To investigate the change of biological characters under the hypoxic environment in vitro in osteosarcoma cell line SaOS-2. Methods The hypoxia culture model was established by a hypoxic chamber. Flow cytometer was used to analyze the cell cycles. The ability of invasion and migration in vitro was detected by scratch injury model and Boyden chamber. The gene productions of VEGF were observed at different hypoxic culture phases by the semi-quantitative reverse transcription PCR（ RTPCR） and immunohistochemical staining. Results Within 16 h of hypoxic exposure, cells were arrested in Gl phase. After 24 h hypoxic exposure, the cycle arrest was relieved. The ability of invasion and migration in SaOS-2 cells was increased significantly after hypoxic culture. After the hypoxia treatment, the expression levels of VEGF mRNA and protein was up-regnlated under hypoxia. Conclusion There was a hypoxia tolerance mechanism in osteosarcoma cells. Osteosarcoma cell could accommodate itself to the hypoxic environment by a series of biological changes.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2007年第6期688-689,共2页
Chinese Journal of Experimental Surgery
关键词
肉瘤
缺氧
耐受
Osteosarcoma
Hypoxia
Tolerance
