摘要
从刀豆素A刺激12 h和15 h的巴马小型猪外周血淋巴细胞中提取总RNA,用RT-PCR方法扩增出β-干扰素(IFN-β)基因,将其克隆到PMD18-T载体上,进行序列分析和蛋白结构预测。结果表明,克隆的巴马猪IFN-β基因氨基酸序列与GenBank上登录的梅山猪和长白猪的IFN-β基因氨基酸同源性为98.4%和98.9%,与人、牛、马的IFN-β氨基酸同源性分别为65.1%,65.1%,66.1%。人和猪IFN-β基因在5个螺旋结构序列上同源性很高,而且在维持蛋白结构和生物学活性上的氨基酸位点也非常保守。
The lymphocytes of Bama mini pig were separated from peripheral blood and the total RNA was extracted from the peripheral blood lymphocytes after 12 hours and 15 hours of stimulation with ConA, respectively. The interferon β （IFN--v） gene was amplified by reverse transcription polymerase chain reaction （RT--PCR） and cloned into PMD18-T vector, and then sequenced. The deduced amino acid sequence analysis of IFN-β gene of Bama mini pig showed that it shared 98. 4%- 98. 9% homology with corresponding pig genes registered in GenBank, and 65.1% ,65.1% and 66.1% similarity with those of human,bovine and horse,respectively. The identity of five helix structure sequences of IFN-β were similar between human and pig. The amino acid loci that maintain protein structure and biological activity were also conservative.
出处
《河南农业科学》
CSCD
北大核心
2007年第5期105-108,共4页
Journal of Henan Agricultural Sciences
关键词
巴马小型猪
β-干扰素基因
克隆
序列分析
结构预测
Bama mini pig
Interferon β gene
Cloning
Sequence analysis
Predicted protein structure
