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应用磁珠法和巢式PCR检测血清HIV_1RNA的临床研究

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摘要 目的探讨磁珠法提取核酸和巢式核酸扩增HIV1RNA在HIV感染诊断中的应用价值。方法对HIV1+2抗体阴性的梅毒感染者、有输血/手术史的住院患者和HIV1+2抗体阳性的AIDS患者、HIV携带者、健康体验者采用上述方法检测血清HIV1RNA。结果24例HIV1+2抗体阴性者血清中未检出HIV1RNA;4例AIDS和1例HIV携带者血清HIV1RNA呈阳性,2例健康体检者血清中未检出HIV1RNA。结论磁珠法提取核酸结合巢式核酸扩增能检出AIDS和HIV携带者血清中的HIV1RNA及排除假阳性。
出处 《现代检验医学杂志》 CAS 2007年第3期129-129,共1页 Journal of Modern Laboratory Medicine
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  • 1Schwartz DH, Mazumdar A, Winston S, et al. Utility of various commercially available human immunodeficiency virus (HIV) antibody diagnostic kits for use in conjunction with efficacy trials of HIV-1vaccines. Clin Diagn Lab Immunol, 1995,2: 268-271.
  • 2Ghosh K, Javeri KN, Mohanty D, et al.False-positive serological tests in acute malaria. Br J Biomed Sci, 2001, 58: 20-23.
  • 3Esteva MH, Blasini AM, Ogly D, et al.False positive results for antibody to HIV in two men with systemic lupus erythematosus. Ann Rheum Dis, 1992, 51:1071-1073.
  • 4Myrmel H, Haukenes G. False-positive band in the gp41 region in anti-HIV western blotting. APMIS, 1988, 96: 950-951.
  • 5Simonsen L, Buffington J, Shapiro CN, et al. Multiple false reactions in viral antibody screening assays after influenza vaccination. Am J Epidemiol, 1995, 141:1089-1096.
  • 6Walensky RP, Rosenberg ES, Ferraro MJ,et al. Investigation of primary human immunodeficiency virus infection in patients who test positive for heterophile antibody.Clin Infect Dis, 2001, 33: 570-572.
  • 7Centers for Disease Control and Prevention. Update: HIV-2 infection among blood and plasma donors-United States, June 1992-June 1995. MMWR, 1995, 42: 603-606.
  • 8Centers for Disease Control and Prevention. Identifieation of HIV-1 Group O infection-Los Angeles County, California,1996. MMWR, 1996, 45: 561-564.
  • 9Migali E, Mariotti D, Lovari A, et al.HIV-1: absence of infection in subjects with indeterminate western blot. Allergol Immanopathol (Madr), 1993, 21: 61-65.
  • 10Zaaijer HL, van Rixel T, van Exel-Oehlers P, et al. New anti-human immunodefieieney virus immunoblot assays resolve nonspecific western blot results. Transfusion,1997, 37: 193-198.

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