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抗SARS-CoV病毒N蛋白的单链抗体(scFv)筛选 被引量：2

Screen of human single chain antibody against N protein of SARS-CoV
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摘要 目的筛选出抗SARS-CoV病毒N蛋白的单链抗体。方法利用原核表达所获得的SARS病毒N蛋白,筛选人源单链抗体噬菌体展示库,经特异性的检测,以期得到抗SARS-CoV病毒N蛋白的特异单链抗体。结果获得了8个抗SARS病毒N蛋白的候选克隆。经测序,获得了编码抗体可变区的基因序列,并进行了原核表达。结论筛选得到的抗SARS-CoV病毒N蛋白单链抗体具有高度的特异性,可以用作临床实验或研究SARS病毒过程中快速检测SARSN蛋白或SARS病毒粒子的候选抗体。 Objective To prepare the single chain antibody against N protein of SARS- CoV. Methods With N protein of SARS- CoV expressed in E. coli as antigen, we obtained the single chain antibody against N protein by screening the phage display library of human single chain antibodies. Results The anti-N protein antibody didn＇t crossinteracte with BSA and the short peptide containing 6 histidines. The specific interaction between the antibody and N protein was inhibited by the anti-N protein monoclone antibody from immunized mice. Conclusion The single chain antibody we got is specific to N protein of SARS-CoV, it can be a candidate antibody for fast detection of N protein of SARS-CoV and SAILS virus particles in clinical trial study of SAILS pathogenesis.
作者 师珍艳 阴彬 魏群 彭小忠
机构地区 北京师范大学生命科学学院生物化学与分子生物学教研室 中国医学科学院中国协和医科大学基础医学研究所
出处 《基础医学与临床》 CSCD 北大核心 2007年第6期674-679,共6页 Basic and Clinical Medicine
关键词 SARS-CoV病毒 N蛋白 人源单链抗体噬菌体展示 SARS-CoV, nucleocapsid protein, phage display library of human single chain antibodies
分类号 R392 [医药卫生—免疫学]
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共引文献24

同被引文献21

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基础医学与临床
2007年 第6期

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