摘要
背景:研究表明骨折端一定形式的微动可以促进骨痂的形成,但微动影响骨折愈合的分子生物学机制还不明确。目的:研究骨折端微动时应力对断端碱性成纤维细胞生长因子的影响。设计:随机对照的动物实验。单位:解放军第二炮兵总医院骨科,解放军总医院第一附属医院骨科,解放军总医院第一附属医院药剂科。材料:实验于2003-03/2004-04在解放军总医院第一附属医院动物实验室及解放军军事医学科学院八室完成。选取健康纯种新西兰大耳白兔72只,清洁级,月龄5～6个月,体质量2.5～3.0kg,由军事医学科学院动物中心提供。按随机数字表法分为两组:微动组和固定组,每组36只。两组动物又分别分为术后7,14,21,28,42,56d6个时间点进行观察,每个时间点6只。方法:应用氯胺酮、速眠新肌注麻醉所有动物,于胫骨平台下3,3.5,5.5,6cm分别旋入固定针,安装外固定架,夹具距内侧骨皮质1.5cm,胫骨平台下4.5cm处横行截断胫骨,固定组、微动组骨折间隙分别为2.0,2.5mm。固定组动物应用单臂外固定架固定,解剖复位骨折端。微动组动物截骨固定后使外固定架中间杆有0.5mm的轴向移动。术后动物自由行走,依靠自身体质量使外固定架产生微动。术后7,14,21,28,42,56d处死动物。以骨折端为中心,切取1cm长标本,分割,固定12h。术后7,14,21,28,42,56d采用免疫组织化学染色和JVC图像分析处理系统行碱性成纤维细胞生长因子定量分析和显色强度判定。主要观察指标:①碱性成纤维细胞生长因子显色强度判定;②碱性成纤维细胞生长因子定量分析。结果:纳入72只白兔均进入结果分析。碱性成纤维细胞生长因子存在于间质细胞、血管内皮细胞、成骨细胞、成软骨细胞、骨细胞胞浆内表达。术后14,21,28d微动组碱性成纤维细胞生长因子蛋白阳性指数分别为1.98±0.14,2.04±0.12,2.13±0.17,明显大于固定组(1.59±0.14,1.68±0.15,1.63±0.27,P<0.05)。结论:微动应力可使骨折端碱性成纤维细胞生长因子增多,促进骨折愈合。
BACKGROUND： Some researches demonstrate that jog stress at fracture part can promote formation of callus, but molecular biological mechanism of jog stress on fracture healing is still unclear.
OBJECTIVE： To study the effect of jog stress at fracture site on basic fibroblast growth factor （bFGF） at broken parts
DESIGN ： Randomized controlled animal study
SETTING： Department of Orthopaedics, General Hospftal of the Second Artilleryman of Chinese PLA; Departments of Orthopaedics and Pharmacy, the First Affiliated Hospital of General Hospital of Chinese PLA,
MATERIALS ： The experiment was carried out in the Animal Laboratory, the First Affiliated Hospital of General Hospital of Chinese PLA and the 8th Laboratory, Academy of Military Medical Sciences of Chinese PLA from March 2003 to April 2004, A total of 72 healthy purebred New Zealand rabbits, of general grade, 5-6 months old, weighing 2.5-3.0 kg, were provided by Animal Center of Academy of Military Medical Sciences of Chinese PLA. All rabbits were randomly divided into jog group and fixation group with 36 rabbits in each group. And then, every 6 rabbits in both groups were respectively observed at 6 time points, including 7, 14, 21, 28, 42 and 56 days.
METHODS ： All animals were anesthetized with ketamine and sumian xinji. Fixation needle was rotated into 3, 3.5, 5.5 and 6 cm below platform of tibia, respectively, and then external fixators were installed. Chuck which was located at 1.5 cm from internal cortical bone and 4.5 cm below platform of tibia crossly cut off tibia. Fracture space was 2.0 mm and 2.5 mm in the fixation group and jog group, respectively. Animals in the fixation group were fixed with unilateral external fixator to dissect replace fracture part. In addition, animals in the jog group cut off their bone, fixed, and moved 0.5 mm to axis along central staff Of external fixator. Animals were able to freely walk and external fixator was slightly shaken based on body mass of animal. Furthermore, all animals were sacrificed at 7, 14, 21, 28, 42 and 56 days after operation. According to the center of fracture part, samples in 1 cm length were cut, divided and fixed for 12 hours. Quantitative analysis of bFGF and colorized intensity were detected with immunohistochemical stain and JVC imaging analytic system at 7, 14, 21, 28, 42 and 56 days after operation.
MAIN OUTCOME MEASURES ： （1） Detecting colored intensity of bFGF; （2） quantitative analysis of bFGF
RESULTS： A total of 72 rabbits were involved in the final analysis, bFGF could express in interstitial cells, vascular endothelial cell, osteoblast, chondroblast and osteocyte plasma. At 14, 21 and 28 days after operation, positive indexes of bFGF protein in the jog group were 1.98±0.14, 2.04±0.12 and 2.13±0.17, respectively, which were obviously higher than those in fixation group （1.59±0.14, 1.68±0.15, 1.63±0.27, P 〈 0.05）.
CONCLUSION ： Jog stress can increase numbers of bFGF at fracture part and promote fracture healing
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第19期3858-3860,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
