摘要
利用响应面优化法对盾叶薯蓣块茎中薯蓣皂甙元水解原位提取进行研究。实验采用CC0428中心组合设计考察了影响薯蓣皂甙元提取效果的四个重要因素:硫酸浓度、异丙醇浓度、水解时间和水解液用量。结果表明盾叶薯蓣块茎中薯蓣皂甙元水解原位提取最佳条件为1.50g盾叶薯蓣块茎干粉加入25mL1.25mol·L-1硫酸-70%异丙醇溶液加热回流水解提取7.5h,薯蓣皂甙元理论产率为0.7220%;实际产率为0.7034%。与传统盐酸水解法相比薯蓣皂甙元产率提高68%。提取的产物通过熔点测定、红外光谱和质谱分析,表明与薯蓣皂甙元标准品基本一致。水解原位提取法具有简便、高效特点。
The process of simultaneous extraction and hydrolysis in situ for the recovery of diosgenin from rhizomes of Dioscorea zingiberensis was optimized by response surface methodology （RSM）. The four parameters of the process, namely, sulfuric acid concentration, isopropanol concentration, hydrolysis time and hydrolysis volume affect the maximum yield of diosgenin significantly and were studied. A central composite design （CCD） involving 28 experiments of four variables at five levels was employed to analyze the recovery process of diosgenin. The results show that the optimum values of above four parameters for achieving maximum yield of diosgenin are as follows： the sulfuric acid concentration of 1.25 mol.L-I, isopropanol concentration of 70%, hydrolysis time of 5 h and hydrolysis volume of 25 mL per 1.50 g pulverized rhizomes, under those conditions, the theoretical yield is 0.7220% and the experimental yield is 0.7034%. Using the process of simultaneous extraction and hydrolysis in situ, the recovery of diosgenin increases 68% comparing with the diosgenin yield of hydrolysis method with aqueous hydrochloric acid. Moreover, the product of the hydrolysis in situ was characterized by melting point, IR and MS. The experiments show that the hydrolysis in situ method is simple and convenient.
出处
《高校化学工程学报》
EI
CAS
CSCD
北大核心
2007年第3期538-542,共5页
Journal of Chemical Engineering of Chinese Universities
基金
国家自然科学基金(20276030)
关键词
薯蓣皂甙元
盾叶薯蓣
水解原位提取
中心组合设计-响应面优化
diosgenin
Dioscorea zingiberensis
simultaneous extraction and hydrolysis in situ
a central composite design-response surface methodology（CCD-RSM）
