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原代大鼠边缘群肝细胞的分离及端粒酶活性分析 被引量：1

Isolation of side population of primary rat hepatic cells and analysis of its telomerase activity
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摘要 目的:通过检测端粒酶活性(TMA)在原代大鼠边缘群及非边缘群肝细胞中表达差异,探讨边缘群细胞是否具有肝脏干细胞的部分特征.方法:原位两步胶原酶法分离大鼠肝细胞.应用流式荧光激活细胞分选术(FACS)将肝细胞分成边缘群(SP)及非边缘群(Non-SP),并定量分析两群细胞中TMA水平.结果:存在SP细胞并占细胞总体2.1%,SP细胞TMA高于Non-SP细胞(28.1±1.3vs2.0±0.2TPG,P<0.01).结论:大鼠边缘群肝细胞具有更强的自我更新能力,即具有干细胞的部分特征. AIM： To detect the telomerase activity in side population （SP） and Non-SP of primary rat hepatic cells so as to explore whether the SP hepatic cells express some characteristics of liver stem cells. METHODS： Primary rat hepatic cells were isolated by two-step /n situ collagenase perfusion. SP cells were identified by fluorescence-activated cell sorting analysis. Telomerase activity （TMA） in SP and Non-SP cells was quantitated. RESULTS： SP cells were present and accounted for 2. 1% of primary rat hepatic cells. TMA was much more expressed in SP cells （28.1 ±1.3 vs2.0±0.2, P〈0.01）. CONCLUSION： SP of primary rat hepatic cells has stronger self-renewal capability and possesses some characteristics of stem cells.
作者 刘海亮 窦科峰 李韧 李霄 张福琴
机构地区 第四军医大学西京医院肝胆外科
出处 《第四军医大学学报》 北大核心 2007年第11期1021-1023,共3页 Journal of the Fourth Military Medical University
关键词 肝细胞 大鼠 边缘群 端粒酶 干细胞 hepatic cells rat side population telomerase stem cells
分类号 R318 [医药卫生—生物医学工程]
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参考文献8

  • 1Dunn JC,Tompkins RG,Yarmush ML.Long-term in vitro function of adult hepatocytes in a collagen sandwich configuration[J].Biotechnol Prog,1991,7(3):237-245.
  • 2Flores I,Benetti R,Blasco MA.Telomerase regulation and stem cell behaviour[J].Curr Opin Cell Biol,2006,18(3):254-260.
  • 3Masutomi K,Yu EY,Khurts S.Telomerase maintains telomere structure in normal human cells[J].Cell,2003,114(2):241-253.
  • 4Guo Y,Follo M,Geiger K,et al.Side population cells from different precursor compartments[J].J Hematother Stem Cell Res,2003,12(1):71-82.
  • 5Preffer FI,Dombkowski D,Sykes M,et al.Lineage-negative side population cells with restricted hematopoietic capacity circulate in normal human adult blood:Immunophenotypic and functional characterization[J].Stem Cells,2002,20(5):417-427.
  • 6Hadnagy A,Gaboury L,Beaulieu R,et al.SP analysis may be used to identify cancer stem cell populations[J].Exp Cell Res,2006,312(19):3701-3710.
  • 7Shimano K,Satake M,Okaya A,et al.Hepatic oval cells have the side population phenotype defined by expression of ATP-binding cassette transporter ABCG2/BCRP1[J].Am J Pathol,2003,163(1):3-9.
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同被引文献11

  • 1张宁,窦科峰,李韧,樊菁,张福琴.肝癌细胞系MHCC97亚群中甲胎蛋白的表达差异[J].第四军医大学学报,2006,27(19):1799-1801. 被引量：7
  • 2Crosby HA, Kelly DA, Strain AJ. Human hepatic stem-like cells isolated using c-kit or CD34 can differentiate into biliary epithelium [J]. Gastroenterology, 2001, 120(2): 534-544.
  • 3Parent R, Marion MJ, Furio L, et al. Origin and characterization of a human bipotent liver progenitor cell line [ J ]. Gastroenterology, 2004,126(4) : 1147 -1156.
  • 4Tanaka S, Yamamoto T, Tanaka H, et al. Potentiality of combined hepatocellular and intrahepatic cholangiocellular carcinoma originating from a hepatic precursor cell: Immunohistochemical evidence [J]. Hepatol Res, 2005,32(1): 52-57.
  • 5Yamamoto T, Uenishi T, Ogawa M, et al. Immunohistologic attempt to find carcinogenesis from hepatic progenitor cell in hepatocellular carcinoma[J]. Dig Surg, 2005,22(5): 364-370.
  • 6Goodell MA, Brose K, Paradis G, et al. Isolation and functional properties of routine hematopoietic stem cells that are replicating in vivo[J]. Exp Med, 1996,183(4): 1797-1806.
  • 7Guo Y, Folio M,Geiger K,et al. Side-population cells from different precursor compartments [ J ]. Hematother Stem Cell Res, 2003, 12(1) : 71 -82.
  • 8Uchida N, Fujisaki T, Eaves AC, et al. Transplantable hematopoietic stem cells in human fetal liver have a CD34 ( + ) side population (SP)phenotype[J]. Clin Invest, 2001, 108(7) : 1071 -1077.
  • 9Preffer FI, Dombkowski D, Sykes M, et al. Lineage-negative sidepopulation (SP) cells with restricted hematopoietic capacity circulate in normal human adult blood: immunophenotypic and functional characterization[ J]. Stem Cells, 2002,20 (5) : 417 - 427.
  • 10张宁,窦科峰,李韧,樊菁,张福琴.甲胎蛋白在肝癌细胞系HepG2不同亚群中的差异表达分析[J].中华肝胆外科杂志,2007,13(10):713-714. 被引量：2

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第四军医大学学报
2007年 第11期

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