摘要
目的:探讨盆腔子宫内膜异位症患者外周血和腹腔液NK细胞表面自然细胞毒受体的表达及意义。方法:用流式细胞术直标法检测20例盆腔子宫内膜异位症患者和13例非子宫内膜异位症对照者外周血和腹腔液NK细胞表面自然细胞毒受体NKp30、NKp44和NKp46的表达。结果:(1)NKp30在腹腔液CD56+NK细胞表面表达观察组较对照组明显减少(P<0.05),在腹腔液CD16+NK细胞表面和外周血CD56+/CD16+NK细胞表面表达两组之间则无差异;NKp46在外周血和腹腔液CD56+/CD16+NK细胞表面表达观察组和对照组之间无显著差异;NKp44在外周血和腹腔液CD56+/CD16+NK细胞表面均无表达;(2)NKp30在腹腔液CD56+CD16+NK细胞上表达观察组比对照组明显减少;外周血CD56+CD16+NK细胞表面NKp30表达以及外周血和腹腔液CD56+CD16+NK细胞表面NKp46表达观察组和对照组之间则无差异;(3)NKp30和NKp46在腹腔液CD16+NK细胞表面表达较外周血显著降低(PNKp30<0.05;PNKp46<0.01),而CD56+NK细胞表面外周血和腹腔液之间的表达则无差异。结论:腹腔液NK细胞表面自然细胞毒受体NKp30在盆腔子宫内膜异位症发病过程中起作用。
Objective： To investigate the expression of natural cytotoxicity receptor （NCR） on NK cells in peripheral blood and peritoneal fluid with or without endometriosis. Methods： NCRs, including NKp30、NKp44 and NKp46, on NK cells in peripheral blood and peritoneal fluid of 20 patients with endometriosis and 13 women without endometriosis were examined with flow cytometry. Results： （ 1 ） The expression of NKp30 on CD56^＋ NK cells in peritoneal fluid of the case group was found to be lower than that of the control group （ P 〈 0.05 ）, whereas there had no significant difference of the expression of NKp30 on CD16^＋ NK cells in peritoneal fluid and on CD56^＋/CD16^＋ NK cells in peripheral blood between these two groups. There was no significant difference of the expression of NKp46 on CD56^＋/CD16^＋ NK cells in peripheral blood or peritoneal fluid between the case group and the control group. NKp44 was not expressed on CD56^＋/CD16^＋ NK cells in peripheral blood or peritoneal fluid in all subjects. （2） The expression of NKp30 on CD56^＋CD16^＋ NK cells in peritoneal fluid was decreased within the case group. （3）The expressions of NKp30 and NKp46 on CD16^＋ NK cells in peritoneal fluid were lower than those in peripheral blood（PNKp30 〈0.05 ;PNKp46 〈0.01 ） ,but there had no difference on CD56^＋ NK cells between peripheral blood and peritoneal fluid. Conclusion：The expression of NKp30 on CD56^＋/CD56^＋CD16^＋ NK cells in peritoneal fluid may play an important role in the pathogenesis of pelvic endometriosis.
出处
《现代妇产科进展》
CSCD
北大核心
2007年第5期358-361,共4页
Progress in Obstetrics and Gynecology
