摘要
Objective： To observe growth inhibition effect of adeno-associated viral vectors （AAV） mediated angiostatin （ANG） gene on implanted breast cancer in rat and its mechanism. Methods： Gene transfer technique was used to transfer AAV-ANG to the tumor. Growth curves were drawn to observe the growth of breast cancer implanted in rat, and immunohistochemical method was used to detect the effects of angiostatin on microvesel density （MVD） of breast cancer implanted in rat. Results： Angiostatin inhibited the growth of breast cancer implanted in rat and decreased the microvessel density of tumor. Conclusion： Expression of an angiostatin transgene can suppress the growth of breast cancer implanted in rat through the inhibition of the growth of microvessels, surggesting that angiostatin gene transfer technique may be effective against breast cancer.
Objective： To observe growth inhibition effect of adeno-associated viral vectors （AAV） mediated angiostatin （ANG） gene on implanted breast cancer in rat and its mechanism. Methods： Gene transfer technique was used to transfer AAV-ANG to the tumor. Growth curves were drawn to observe the growth of breast cancer implanted in rat, and immunohistochemical method was used to detect the effects of angiostatin on microvesel density （MVD） of breast cancer implanted in rat. Results： Angiostatin inhibited the growth of breast cancer implanted in rat and decreased the microvessel density of tumor. Conclusion： Expression of an angiostatin transgene can suppress the growth of breast cancer implanted in rat through the inhibition of the growth of microvessels, surggesting that angiostatin gene transfer technique may be effective against breast cancer.
