摘要
对几种特异性强、灵敏度高的抗人肺癌细胞单克隆抗体（ＭｃＡｂ）及其片段，选择理想的放射性核素标记，为临床放射免疫显像（ＲＩＩ）提供实验依据。采用本室提供的３种ＩｇＧＭｃＡｂ：抗人大细胞肺癌ＭｃＡｂ（２Ｅ３）、抗人肺鳞状细胞癌ＭｃＡｂ（Ｓｍ１）、抗人肺腺癌ＭｃＡｂ（Ａｍ７）及２Ｅ３Ｆ（ａｂ′）２，中国科学院上海细胞所提供的ＩｇＭ抗人肺腺癌ＭｃＡｂ（ＬＣ１）及其片段，制备载人大细胞肺癌细胞株（ＰＬＡ８０１）或人肺腺癌细胞株（沪Ａ１）肿瘤裸鼠模型。注入１２５Ｉ、１３１Ｉ或９９ｍＴｃ标记的上述ＭｃＡｂ，行载瘤裸鼠显像。动态显像时应用感兴趣区法计算各时相Ｔ／ＮＴ值，并在注射ＭｃＡｂ后２４小时杀死动物，测定抗体生物分布。结果１３１Ｉ标记Ａｍ７、Ｓｍ１、２Ｅ３及ＬＣ１在２４小时载瘤裸鼠显影均良好。动态显像，各时相Ｔ／ＮＴ值及２４小时生物分布表明：９９ｍＴｃ标记ＭｃＡｂ优于１３１Ｉ标记ＭｃＡｂ；抗体片段优于完整抗体；单抗在同型抗原肿瘤的浓聚高于异型抗原肿瘤。故临床肿瘤ＲＩＩ核素采用９９ｍＴｃ、抗体选择混合抗体为好。
PURPOSE To improve radioimmunoimaging (RII) by using highly specific and sensitive antihuman lung cancer cell monoclonal antibodies (McAb) and their fragments and selecting a desirable radionuclide for labelling METHODS ①Preparation of nude mice bearing human lung cancer cell lines (PLA 801 or Hu A 1) xenografts ②Labelling of three kinds of IgG McAb (2E 3 , Sm 1, Am 7), 2E 3 F(ab') 2 and IgM McAb (LC 1) and its fragment with radioiodine or 99m Tc ③Performing RII of these McAbs and their fragments by nude mice bearing xenografts ④Measuring of the in vivo T/NT values using ROI technique and in vitro T/NT values 24 h after injection of radiolabeled McAbs RESULTS ①Good images were obtained in animal models after injection of all types of radiolabelled antibodies ②In vivo and in vitro T/NT values showed that 99m Tc labelling was better than radioiodine labelling and fragments were superior to intact McAbs ③The uptake of 131 I labelled LC 1 fragments in Hu A 1 xenografts was higher than that in PLA 801 xenograftes CONCLUSIONS This study lays the basis for using 99m Tc labelling and a mixture of antibodies in the clinical diagnosis of lung cancer by RII
出处
《中华核医学杂志》
CAS
CSCD
北大核心
1997年第1期46-48,共3页
Chinese Journal of Nuclear Medicine
基金
"八五"国家重点科技攻关项目
关键词
单克隆抗体
肺肿瘤
放射免疫显像
Antibodies, monoclonal Antibodies, neoplasm Lung neoplasms Radioimmunoimaging Animals, laboratory
