摘要
本文探讨了检测牛组织（血浆、肌肉和肝）中阿维菌素（ＡＶＭ）残留的间接竞争ＥＬＩＳＡ反应条件和样本前处理方法，并对添加样本进行了测定。竞争反应可耐受２０％（ｖ／ｖ）的甲醇。使用４″－Ｏ－（单）琥珀酰ＡＶＭ－ＯＶＡ和５－Ｏ－（单）琥珀酰ＡＶＭ－ＯＶＡ作包被原，ＡＶＭ标准液的Ｉ５０＝１．８～４．０ｎｇ／ｍＬ，检测极限为０．０２～０．２ｎｇ／ｍＬ。样本经甲醇一次提取和稀释后直接进行检测，各样本介质中ＡＶＭ的Ｉ５０＝３．６～３０．２ｎｇ／ｍＬ，样本检测极限为０．１～０．６μｇ／ｋｇ。在６．０～６０．０μｇ／ｋｇ添加浓度范围内，回收率（％）＝８７．９～１０８．８（９５．０±８．０），变异系数（ＣＶ．％）＝２．８～１６．９（１０．０±５．２）。本方法适宜对大批量样本进行快速检测。
A rapid and sensitive indirect competitive ELISA for avermectin B 1(AVM)residue was developed.The competitive conditions of ELISA were optimized in pH values,ionic strengths,and organic solvent.The ELISA can tolerate 20%(v/v)methanol.In PBS 0.5% Tween 20,the limits of detection for different C Ag were 0.02～0.2ng/ml for AVM,with half maximal inhibition(I 50 )1.8～4.0ng/ml.Samples (cattle plasma,muscle and liver)were extracted with methanol,and the extracts were determined by ELISA after diluted with PBS-0.5% Tween 20(1∶1,v/v).The I 50 of standard curve for AVM ranged from 3.6 to 30.2ng/ml for different samples.Limits of detection for AVM were 0.1～0.6μg/kg.At 6.0～60.0μg/kg spiking levels,Recoveries(%)were 87.9～108.8(95.0±8.0),and CV(%)were 2.8～16.9(10.0±5.2).This can be used as a rapid method for detection of AVM in animal tissues.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1997年第1期77-83,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
关键词
牛
阿维菌素
组织残留
酶联免疫吸附
残留
Avermectin B 1,Enzyme-linked immunosorbent assay(ELISA),Residue analysis
