摘要
以建立的传染性法氏囊病病毒（ＩＢＤＶ）逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）和核酸杂交检测方法，检测了细胞培养物、病理组织、粪便和饲料中的ＩＢＤＶ，并与ＩＢＤＶ夹心ＥＬＩＳＡ、琼脂扩散试验（ＩＤ）和病毒分离方法进行了平行比较试验。结果表明，ＲＴ－ＰＣＲ和核酸杂交检测方法具有高度的敏感性和特异性，是深入研究ＩＢＤ流行病学。
The methods for the detection of infectious bursal disease virus (IBDV) with reverse transcribed polymerase chain reaction (RT PCR) and DNA dot blot hybridization were established and used to detect the samples of tissue culture, pathological tissues, feces and feeds. Compared with methods of IBDV sandwich ELISA, immunodiffusion test (ID) and virus separation, RT PCR and DNA hybridization were more sensitive and specific for the survey of epidemiology, especially the route of transmission of IBDV.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1997年第1期12-14,共3页
Chinese Journal of Veterinary Science
